| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,598,488 | C→T | 32.6% | A60A (GCG→GCA) | yfeT ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,598,488 | 0 | C | T | 32.6% | 61.5 / 40.5 | 49 | A60A (GCG→GCA) | yfeT | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (21/12); new base T (7/9); total (28/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
TTCGTTTTCTCCCGGCTGGCACTGTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTGTTTCGCCATCTGCCGT > minE/1598423‑1598557 | ttCGTTTTCTCCCGGCTGGCACTGTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGCGCGcc < 1:54965/71‑1 (MQ=255)ttCGTTTTCTCCCGGCTGGAACTGTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGCGCGcc > 1:652318/1‑71 (MQ=255) cGTTTTCTCCCGGCTGGCACTGTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGCGCGcc > 1:644136/1‑69 (MQ=255) cGTTTTCTCCCGGCTGGCACTGTATTCGCCTATTAACGCAATACGCAATTCAGTAAATCCTTGCGCGcc > 1:242693/1‑69 (MQ=255) gTTTTCTCCCGGCTGGCACTGTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGTGCGCCGAg > 1:1667053/1‑71 (MQ=255) gTTTTCTCCCGGCTGGCACTGTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGTGCGCCGAg > 1:387372/1‑71 (MQ=255) ttttCTCCCGGCTGGCACTGTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGCGCGCCg < 1:2035217/68‑1 (MQ=255) ttCTCCCGGCTGGCACTGTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGTGCGCCGAGtt < 1:1661511/70‑1 (MQ=255) ctcCCGGCTGGCACTGTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGTGCGCCGAGtttt < 1:1323244/70‑1 (MQ=255) ggCTGGCACTGTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGTGCGCCGAGTTTTTGGGCa < 1:400285/71‑1 (MQ=255) gTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGTGCGCCGa > 1:594534/1‑50 (MQ=255) tATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGTGCGCCGAGttttt < 1:857041/55‑1 (MQ=255) tATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTAt > 1:808280/1‑71 (MQ=255) ttCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGc > 1:1594205/1‑71 (MQ=255) cGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTggg > 1:1298062/1‑54 (MQ=255) gCCTATTAACGCCATACGCAATTCAGTAAATCCTTGTGCGCCGAGTTTTTGGGCAAATTTCACTATGCTc < 1:1031940/70‑1 (MQ=255) gCCTATTAACGCCATACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCg < 1:1527550/71‑1 (MQ=255) gCCTATTAACGCCATACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCg < 1:1202408/71‑1 (MQ=255) ttAACGCCATACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCAc < 1:575947/58‑1 (MQ=255) aaCGCCATACGCAATTCATTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATg > 1:318937/1‑60 (MQ=255) aaCGCCATACGCAATTCAGTAAATCCTTGTGCGACGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGc > 1:260195/1‑70 (MQ=255) aCGCCATACGCAATTCAGTAAATCCTTGTGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCt < 1:279217/70‑1 (MQ=255) aCGCCATACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCt > 1:1488694/1‑70 (MQ=255) aCGCCATACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCt > 1:1528322/1‑70 (MQ=255) aCGCCATACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCt > 1:340674/1‑70 (MQ=255) gCCATACGCAATTCAGTAAATCCTTGTGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCt < 1:1742297/68‑1 (MQ=255) aTACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGcc < 1:1029936/71‑1 (MQ=255) tACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCAc < 1:387358/49‑1 (MQ=255) aCGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGcc > 1:1637192/1‑69 (MQ=255) cGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAAt > 1:1329833/1‑42 (MQ=255) cGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCa > 1:2056528/1‑46 (MQ=255) cGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCa > 1:1896349/1‑46 (MQ=255) cGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCa > 1:1551716/1‑46 (MQ=255) cGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGa > 1:949247/1‑56 (MQ=255) aaTTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTg < 1:2035718/71‑1 (MQ=255) aaTTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTg > 1:265017/1‑71 (MQ=255) aaTTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCAGAGCTg > 1:1290510/1‑71 (MQ=255) aTTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCa < 1:842495/42‑1 (MQ=255) tCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACt > 1:1000409/1‑42 (MQ=255) cAGTAAATCCTTGTGCGCCGAGTTTTTGGGCAAATTTCACTATGc < 1:383022/45‑1 (MQ=255) cAGTAAATCCTTGTGCGCCGAGTTTTTGGGCAAATTTCACTATGc < 1:771954/45‑1 (MQ=255) aGTAAATCCTTGTGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGAc > 1:2205870/1‑49 (MQ=255) tAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTGTTTCGc > 1:412993/1‑70 (MQ=255) aaTCCTTGTGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTGTTTCGCCAt > 1:125185/1‑71 (MQ=255) aaTCCTTGTGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTGTTTCGCCAt > 1:1656908/1‑71 (MQ=255) aaTCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTAt > 1:1548719/1‑38 (MQ=255) aaTCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTGtt < 1:428449/64‑1 (MQ=255) aaTCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTGtt < 1:1787549/64‑1 (MQ=255) cTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTGTTTCGCCATCTGc < 1:1104780/71‑1 (MQ=255) gcgcgcCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTGTTTCGCCATCTGcc < 1:1483789/69‑1 (MQ=255) cgcgcCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTGTTTCGCCATCTGCCGt < 1:1205891/70‑1 (MQ=255) | TTCGTTTTCTCCCGGCTGGCACTGTATTCGCCTATTAACGCCATACGCAATTCAGTAAATCCTTGCGCGCCGAGTTTTTGGGCAAATTTCACTATGCTCGACTGGCTAATGCCGAGCTGTTTCGCCATCTGCCGT > minE/1598423‑1598557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |