| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,643,860 | A→G | 26.8% | R9R (CGT→CGC) | purC ← | phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,643,860 | 0 | A | G | 26.8% | 82.7 / 32.5 | 51 | R9R (CGT→CGC) | purC | phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
| Reads supporting (aligned to +/- strand): ref base A (13/24); new base G (7/6); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCTT > minE/1643791‑1643926 | cGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCAc < 1:1588269/71‑1 (MQ=255) gTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACcgcg < 1:1861304/71‑1 (MQ=255) gTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCAc < 1:1117565/70‑1 (MQ=255) tATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGa > 1:1332969/1‑71 (MQ=255) tCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACcgcg < 1:363138/68‑1 (MQ=255) aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATAc > 1:798737/1‑70 (MQ=255) aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATAc < 1:1863382/70‑1 (MQ=255) aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATAc > 1:313957/1‑70 (MQ=255) aTTCGAGCACCAACAGGTCCGGGTTTTCCGTTCTGTATACGGTTTTCGCTTTACCACg < 1:952215/58‑1 (MQ=255) aTTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACg < 1:471719/58‑1 (MQ=255) aGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAgcttgc < 1:646647/69‑1 (MQ=255) gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCttt > 1:1270773/1‑71 (MQ=255) gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCttt > 1:1549920/1‑71 (MQ=255) cAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCAc < 1:863743/47‑1 (MQ=255) cAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAgcttgct < 1:1534442/65‑1 (MQ=255) cAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCa < 1:2142210/71‑1 (MQ=255) cAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCa < 1:1141701/71‑1 (MQ=255) cAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCa < 1:1716930/71‑1 (MQ=255) aaCAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACa > 1:1023249/1‑52 (MQ=255) aaCAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGa > 1:613048/1‑48 (MQ=255) aaCAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTAC‑‑CGATACAACTCAGCTTGCTTTTGCATCt > 1:1515664/1‑71 (MQ=255) aCAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCAt > 1:483284/1‑70 (MQ=255) aGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCtt < 1:1754235/71‑1 (MQ=255) tCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt < 1:2091631/71‑1 (MQ=255) tCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTAt < 1:1124471/71‑1 (MQ=255) gTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAgc < 1:222922/48‑1 (MQ=255) ttttCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCt < 1:839773/61‑1 (MQ=255) tttCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCtttt > 1:1849371/1‑54 (MQ=255) ttCCGTACTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTg > 1:282281/1‑71 (MQ=255) cGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTAt < 1:325915/60‑1 (MQ=255) tGCTGTATACGGTTTTCGCTTTACCGCGTTACAACTCAgcttg < 1:1661025/43‑1 (MQ=38) cTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGtgtg < 1:2062144/70‑1 (MQ=255) tataCGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAAtt < 1:225346/71‑1 (MQ=255) taCGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTaa > 1:382649/1‑71 (MQ=255) taCGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATc < 1:1410156/46‑1 (MQ=255) taCGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAAtt < 1:1386275/69‑1 (MQ=255) aCGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAAc > 1:2093117/1‑71 (MQ=255) cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCa > 1:844622/1‑51 (MQ=255) cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCa > 1:742207/1‑51 (MQ=255) cGGTTTTCGCTTTACCACGATACAACTCAgcttgct < 1:2174388/36‑1 (MQ=255) cGGTTTTCGCTTTACCACGATACAACTCAgcttgct < 1:38920/36‑1 (MQ=255) cGGTTTTCGCTTTACCACGATACAACTCAgcttgct < 1:1242199/36‑1 (MQ=255) cGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGaa > 1:1368447/1‑65 (MQ=255) cGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAAc > 1:1648400/1‑70 (MQ=255) cGGTTTTAGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt > 1:1408593/1‑49 (MQ=39) ggTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGc < 1:2183515/40‑1 (MQ=255) ttttCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTTAATTAACGttt > 1:989222/1‑71 (MQ=255) ttCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATc > 1:2061398/1‑45 (MQ=255) tCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTa < 1:717983/70‑1 (MQ=255) tttACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTcc < 1:2201408/45‑1 (MQ=255) tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCt < 1:1057296/70‑1 (MQ=255) tACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc < 1:560663/69‑1 (MQ=255) tACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCtt > 1:1957350/1‑71 (MQ=255) | CGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCTT > minE/1643791‑1643926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |