| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,664,529 | G→A | 36.4% | I231I (ATC→ATT) | guaA ← | GMP synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,664,529 | 0 | G | A | 36.4% | 18.2 / 18.8 | 22 | I231I (ATC→ATT) | guaA | GMP synthetase |
| Reads supporting (aligned to +/- strand): ref base G (9/5); new base A (5/3); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTC > minE/1664462‑1664598 | ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGAt > 1:131753/1‑70 (MQ=255) ccGATAGCGCGGTTCAGCAGCATTGCTGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACtt < 1:1472523/70‑1 (MQ=255) aTAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTAtc > 1:663465/1‑71 (MQ=255) cgcgGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTAtcgtcg > 1:670766/1‑71 (MQ=255) ggTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCt < 1:1787550/71‑1 (MQ=255) gcagcaTTGAGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTgg < 1:1010212/71‑2 (MQ=255) aTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcgcg < 1:2087091/71‑1 (MQ=255) ttGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTc > 1:179265/1‑67 (MQ=255) ttGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACtt > 1:607141/1‑48 (MQ=255) ttGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACtt > 1:1761090/1‑48 (MQ=255) gTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCg < 1:992635/71‑1 (MQ=255) ttACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGa < 1:2034712/71‑1 (MQ=255) tACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAg > 1:1292789/1‑71 (MQ=255) aCGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGcc > 1:607164/1‑50 (MQ=255) cGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGc < 1:1627935/70‑1 (MQ=255) aggaATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTcgcg < 1:1094980/58‑1 (MQ=255) aTCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCa > 1:2102618/1‑70 (MQ=255) cacaCCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTAc > 1:2103405/1‑42 (MQ=255) ccaccaGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAg > 1:2181140/1‑61 (MQ=255) cGAGGATGACTTTATCGTCGCCTACCTGCTCGCgg > 1:1324813/1‑35 (MQ=255) cGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGc > 1:1280348/1‑71 (MQ=255) cGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAAtttt > 1:1581772/1‑64 (MQ=255) ggATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTc < 1:1340555/71‑1 (MQ=255) | TTTTACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGCGTC > minE/1664462‑1664598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |