| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,680,754 | T→C | 31.8% | Y1407C (TAC→TGC) | yfhM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,680,754 | 0 | T | C | 31.8% | 49.0 / 37.0 | 44 | Y1407C (TAC→TGC) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (18/12); new base C (7/7); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-02 | |||||||||||
ACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTGAAC > minE/1680690‑1680823 | aCTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGc > 1:2026805/1‑70 (MQ=255)aCTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCt > 1:1439537/1‑71 (MQ=255)aCTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCt > 1:891930/1‑71 (MQ=255) tttCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCt < 1:1340502/69‑1 (MQ=255) cGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAg > 1:1920102/1‑69 (MQ=255) gATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAg > 1:349897/1‑71 (MQ=255) gATAGACAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAg > 1:367798/1‑71 (MQ=255) aTAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGt > 1:488016/1‑71 (MQ=255) gCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGc < 1:1810335/65‑1 (MQ=255) gCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTAttt > 1:944414/1‑70 (MQ=255) gCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAtttt < 1:958785/71‑1 (MQ=255) ccAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAg > 1:342919/1‑71 (MQ=255) aaaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCa > 1:2081416/1‑68 (MQ=255) aaaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc > 1:506337/1‑70 (MQ=255) aaaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCa < 1:1350392/71‑1 (MQ=255) aaaCGCATGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc > 1:825/1‑70 (MQ=255) aCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAg < 1:252491/71‑1 (MQ=255) ctgctgCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGgaga < 1:813946/71‑1 (MQ=255) gctgcGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGagag > 1:393189/1‑70 (MQ=255) ctgcGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGagag < 1:1165617/69‑1 (MQ=255) gcGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGTTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCAt > 1:750807/1‑70 (MQ=255) gcGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCAt > 1:2090543/1‑70 (MQ=255) cGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTttctt < 1:1495987/53‑1 (MQ=255) cGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCAtt < 1:1281651/70‑1 (MQ=255) gAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTaa > 1:1192433/1‑71 (MQ=255) aaaGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc < 1:1647108/56‑1 (MQ=255) aaGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAAcg < 1:2066762/71‑1 (MQ=255) gtgtgtTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCATCAAGGAGAGCATTAAcgcg > 1:395456/1‑71 (MQ=255) ttCAGCAAAGTGTACTGCTCATCTGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAAcg < 1:1079299/64‑1 (MQ=255) ttCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAAc < 1:2157139/63‑1 (MQ=255) ttCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTc > 1:1982487/1‑71 (MQ=255) ttCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTc > 1:1842898/1‑71 (MQ=255) cAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAAcgc > 1:1916309/1‑63 (MQ=255) cAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcg > 1:237261/1‑70 (MQ=255) aGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCa < 1:1729421/46‑1 (MQ=255) aGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCa < 1:1164958/46‑1 (MQ=255) cAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcgcg > 1:643240/1‑69 (MQ=255) cAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcgcg > 1:994526/1‑69 (MQ=255) aaGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTaa > 1:1976261/1‑55 (MQ=255) aaGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTaa > 1:1702830/1‑55 (MQ=255) aGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCGAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTg < 1:1154992/71‑1 (MQ=255) aGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTg < 1:200321/71‑1 (MQ=255) aGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTg < 1:1805036/71‑1 (MQ=255) aGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTg < 1:1090517/71‑1 (MQ=255) gtgtACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTg > 1:977354/1‑70 (MQ=255) tACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTGAAc < 1:309873/70‑1 (MQ=255) tACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTGAAc < 1:1504272/70‑1 (MQ=255) | ACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTGAAC > minE/1680690‑1680823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |