| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,695,295 | G→A | 37.9% | Q239* (CAG→TAG) | yfhQ ← | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,695,295 | 0 | G | A | 37.9% | 22.4 / 28.0 | 29 | Q239* (CAG→TAG) | yfhQ | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (4/14); new base A (6/5); total (10/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
TAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTA > minE/1695234‑1695362 | tAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCaa < 1:1816466/70‑1 (MQ=255) gCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGc < 1:1203146/71‑1 (MQ=255) ttACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAg < 1:718734/71‑1 (MQ=255) aCGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGaa > 1:602288/1‑71 (MQ=255) gcgTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAg < 1:1424051/67‑1 (MQ=255) aTCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATa < 1:583887/52‑1 (MQ=255) aTCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAAt < 1:181968/51‑1 (MQ=255) tCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCaa < 1:593367/49‑1 (MQ=255) cAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCaa < 1:1874575/48‑1 (MQ=255) gCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAg < 1:1955090/55‑1 (MQ=255) aCGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCaa < 1:78488/42‑1 (MQ=255) aCGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTc < 1:624598/71‑1 (MQ=255) cGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCa < 1:1679561/71‑1 (MQ=255) gCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAAc > 1:1680913/1‑71 (MQ=255) gCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCaa > 1:1425497/1‑39 (MQ=255) gCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCaa > 1:209760/1‑39 (MQ=255) gCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCaa > 1:337713/1‑39 (MQ=255) gCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCaa > 1:446826/1‑39 (MQ=255) ttATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTc < 1:2167374/69‑1 (MQ=255) aTTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGata > 1:1499657/1‑59 (MQ=255) cGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCACAATCCCGCGCAGGATATTCaa > 1:1542151/1‑61 (MQ=255) ttGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCcg < 1:1777049/42‑1 (MQ=255) ttGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCcg < 1:1132944/42‑1 (MQ=255) ttGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCt < 1:1582591/61‑1 (MQ=255) tGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCaa > 1:1206230/1‑55 (MQ=255) tGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCaa > 1:921727/1‑55 (MQ=255) tttATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCtt < 1:192834/58‑1 (MQ=255) ttATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgcg < 1:938833/67‑1 (MQ=255) ttCTACTGCTCAATAGAAGCCAGAAT‑CCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTa < 1:787778/71‑1 (MQ=255) | TAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGTA > minE/1695234‑1695362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |