| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,697,632 | A→G | 17.6% | Y195C (TAT→TGT) | yfhR → | predicted peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,697,632 | 0 | A | G | 17.6% | 111.7 / 20.0 | 51 | Y195C (TAT→TGT) | yfhR | predicted peptidase |
| Reads supporting (aligned to +/- strand): ref base A (20/22); new base G (4/5); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAGCGGCGAAAAT > minE/1697562‑1697702 | cTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTa > 1:1155465/1‑71 (MQ=255)cTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTa > 1:1958783/1‑71 (MQ=255)cTGGATGTTATTGGTCGGGCTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTa > 1:1952377/1‑71 (MQ=255) ggATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTAt > 1:1899678/1‑70 (MQ=255) ttGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAAcc < 1:1421448/68‑1 (MQ=255) ggggTGATCGTGAATGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaac > 1:2016473/1‑71 (MQ=255) ggggTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaac > 1:520267/1‑71 (MQ=255) ggTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca < 1:1947677/71‑1 (MQ=255) ggTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca < 1:1723325/71‑1 (MQ=255) tGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCaaa < 1:2111981/71‑1 (MQ=255) aTCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaaccaa < 1:1046107/68‑1 (MQ=255) aTCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaaccaa < 1:476003/68‑1 (MQ=255) aTCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATg < 1:1052318/71‑1 (MQ=255) tCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCaaa < 1:2130633/68‑1 (MQ=255) tCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGc < 1:2180468/51‑1 (MQ=255) tCGTGAAAGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCaaa < 1:230904/68‑1 (MQ=255) cGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGAt > 1:1310970/1‑71 (MQ=255) aaGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCAt > 1:1565124/1‑52 (MQ=255) aaGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCAt > 1:714537/1‑52 (MQ=255) aGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCAt > 1:1246717/1‑51 (MQ=255) aGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCAt > 1:847013/1‑51 (MQ=255) ggCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCgg < 1:1005538/71‑1 (MQ=255) gCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGc < 1:879349/71‑1 (MQ=255) aTACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAg < 1:745853/71‑1 (MQ=255) aTACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAg < 1:1258869/71‑1 (MQ=255) tACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCAt < 1:1542108/46‑1 (MQ=255) cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAg > 1:250138/1‑68 (MQ=255) cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTgg > 1:1869539/1‑71 (MQ=255) cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTgg > 1:1866996/1‑71 (MQ=255) cGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATAGccaacca > 1:900866/1‑53 (MQ=255) ggTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca < 1:1254246/48‑1 (MQ=255) ggTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca < 1:746517/48‑1 (MQ=255) ggTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca < 1:892667/48‑1 (MQ=255) gTGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCa > 1:460587/1‑61 (MQ=255) tGATCCTCGACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCtactta > 1:1821073/1‑71 (MQ=255) tCCTCGACTCCACATTTGCCTCTTATGCAACCATCGccaacca < 1:1450717/43‑1 (MQ=255) tCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTacttactt < 1:1593014/71‑1 (MQ=255) cTCGACTCCACATTTGCCTCTTGTGCAACCATCGccaaccaa < 1:85092/42‑1 (MQ=255) cGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgat > 1:1155572/1‑70 (MQ=255) gACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgat > 1:265719/1‑69 (MQ=255) gACTCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgat > 1:462372/1‑69 (MQ=255) gACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgatga < 1:231959/71‑1 (MQ=255) gACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgatga < 1:1065747/71‑1 (MQ=255) gACTCCACATTTGCCTCTTATG‑AACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgatga < 1:1340543/70‑1 (MQ=255) aCTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTg < 1:962116/54‑1 (MQ=255) tCCACATTTGCCTCTTGTGCAACCATCGCCAACCAAATg < 1:307240/39‑1 (MQ=255) tCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtgatg > 1:1744803/1‑67 (MQ=255) cacaTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCtacttac > 1:780131/1‑59 (MQ=255) cacaTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTacttactt > 1:545347/1‑61 (MQ=255) acaTTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTa < 1:284642/71‑1 (MQ=255) tttGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCgg > 1:1855531/1‑41 (MQ=255) tttGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCgg > 1:1556266/1‑41 (MQ=255) tctTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATgaga > 1:431660/1‑58 (MQ=255) ctTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAgcggcg > 1:75044/1‑69 (MQ=255) aTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTtga > 1:689597/1‑49 (MQ=255) aTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAGCGGCGaaaa > 1:1908281/1‑70 (MQ=255) aTGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAGCGGCGAAAAt > 1:1580448/1‑71 (MQ=255) | CTGGATGTTATTGGTCGGGGTGATCGTGAAGGCATACGTGCGGTGATCCTCGACTCCACATTTGCCTCTTATGCAACCATCGCCAACCAAATGATCCCCGGCAGTGGCTACTTACTTGATGAGAGTTACAGCGGCGAAAAT > minE/1697562‑1697702 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |