| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,700,462 | A→G | 31.5% | L24P (CTA→CCA) | hcaT ← | predicted 3‑phenylpropionic transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,700,462 | 0 | A | G | 31.5% | 62.8 / 40.0 | 50 | L24P (CTA→CCA) | hcaT | predicted 3‑phenylpropionic transporter |
| Reads supporting (aligned to +/- strand): ref base A (16/18); new base G (5/10); total (21/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAACC > minE/1700402‑1700530 | aaCAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGaaaaa > 1:1386532/1‑67 (MQ=255) cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGt < 1:1438672/71‑1 (MQ=255) cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGt < 1:587364/71‑1 (MQ=255) cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGt < 1:1971871/71‑1 (MQ=255) cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGt < 1:1840501/71‑1 (MQ=255) aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGaaa < 1:541653/62‑1 (MQ=255) gCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAAc > 1:245374/1‑71 (MQ=255) tCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTa > 1:722773/1‑71 (MQ=255) tGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTAtg > 1:2159668/1‑71 (MQ=255) tGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTAtg > 1:2157112/1‑71 (MQ=255) ggCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTAtgt > 1:1444929/1‑71 (MQ=255) cGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTaa > 1:1997381/1‑55 (MQ=255) cGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGa < 1:255657/71‑1 (MQ=255) gTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGa < 1:19939/70‑1 (MQ=255) ttAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGt < 1:587135/51‑1 (MQ=255) ttAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGt < 1:1459885/63‑1 (MQ=255) ttAAACCAATACCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGt < 1:768751/63‑1 (MQ=255) tAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGaaa > 1:250842/1‑70 (MQ=255) aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa > 1:1961262/1‑69 (MQ=255) aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGaaa > 1:545199/1‑69 (MQ=255) aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGaa > 1:1193841/1‑68 (MQ=255) ccTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGACGTAACTAAAAAAGTATGTGAAATAGCCGAgcg > 1:797290/1‑70 (MQ=255) tttAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGcc > 1:1126439/1‑70 (MQ=255) tttAAGCCAGACGCTCCAGAAAGGT‑‑AAAAATGCCGTAACTaa < 1:533534/42‑1 (MQ=38) tAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAAc > 1:1860810/1‑71 (MQ=255) gCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAg < 1:25501/71‑1 (MQ=255) cAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAgcg < 1:591923/71‑1 (MQ=255) aGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGaa < 1:742297/49‑1 (MQ=255) gACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAgcgc > 1:768262/1‑70 (MQ=255) aCGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGt > 1:506149/1‑71 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg < 1:2068467/71‑1 (MQ=255) cTCCAGAAAGGTGGAAAAATGACGTAACTAAAAAAGTATGTGa < 1:1148369/43‑1 (MQ=38) tCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTAtgtg < 1:1461608/41‑1 (MQ=255) tCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTAtgtg < 1:2133681/41‑1 (MQ=255) tCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTAtgtg < 1:1822595/41‑1 (MQ=255) tCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGa < 1:769893/42‑1 (MQ=255) ccAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGa > 1:1130441/1‑69 (MQ=255) ccAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGa > 1:1804574/1‑69 (MQ=255) cAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg < 1:344505/66‑1 (MQ=255) cAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg < 1:1194415/66‑1 (MQ=255) cAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGAt < 1:142503/69‑1 (MQ=255) aGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGa < 1:1931445/39‑1 (MQ=255) gAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGcc > 1:165051/1‑52 (MQ=255) aaaGGTGGAAAAATGCCGTAACTAAAAAAGTAtgtg < 1:724675/36‑1 (MQ=255) aaaGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaa < 1:1610349/71‑1 (MQ=255) aaGGTAGAAAAATGGCGTAACTAAAAAAGTATGTGAAATAGCCGa > 1:747662/1‑45 (MQ=255) aaGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGcc > 1:598886/1‑50 (MQ=255) ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAc < 1:651368/71‑1 (MQ=255) ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAc < 1:894440/71‑1 (MQ=255) gTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAcc < 1:863597/71‑1 (MQ=255) | AACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAACC > minE/1700402‑1700530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |