Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,763,556 T→C 35.5% noncoding (288/1542 nt) rrsG ← 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,763,5560TC35.5% 71.9 / 79.5 76noncoding (288/1542 nt)rrsG16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (26/23);  new base C (13/14);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGC  >  minE/1763502‑1763624
                                                      |                                                                    
ggAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCtt                                                       <  1:1808565/70‑1 (MQ=14)
  aGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGt                                                    <  1:1434455/71‑1 (MQ=34)
      tGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGc                                                >  1:1693325/1‑71 (MQ=21)
        gACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAg                                                 <  1:350113/68‑1 (MQ=35)
        gACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGcc                                               <  1:585996/70‑1 (MQ=25)
        gACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGcc                                               <  1:432341/70‑1 (MQ=25)
         aCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                             <  1:81632/71‑1 (MQ=34)
         aCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                             <  1:1282251/71‑1 (MQ=34)
         aCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                             <  1:322464/71‑1 (MQ=34)
         aCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGt                                             <  1:2018428/71‑1 (MQ=21)
          ccGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGtt                                            <  1:2140732/71‑1 (MQ=34)
          ccGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGtt                                            <  1:99142/71‑1 (MQ=34)
          ccGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGtt                                            <  1:2114527/71‑1 (MQ=34)
          ccGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGtt                                            <  1:916505/71‑1 (MQ=34)
           cGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTa                                           >  1:842022/1‑71 (MQ=34)
             tgtCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAcc                                         <  1:595085/71‑1 (MQ=34)
               tctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTgg                                                     >  1:2025218/1‑57 (MQ=35)
               tctaAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTgg                                                     >  1:1835638/1‑57 (MQ=255)
                 tcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGt                                                    >  1:165940/1‑56 (MQ=255)
                 tcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccac                                     >  1:1948/1‑71 (MQ=21)
                   aGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAcc                                         >  1:1474687/1‑65 (MQ=35)
                       ccAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                 >  1:922074/1‑69 (MQ=40)
                          gtgtGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGc                             >  1:1618193/1‑70 (MQ=255)
                           tgtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGcc                                               <  1:1235735/51‑1 (MQ=35)
                           tgtgGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCt                            >  1:1297258/1‑70 (MQ=255)
                            gtgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                          >  1:517764/1‑71 (MQ=255)
                             tgGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                             >  1:282944/1‑51 (MQ=35)
                               gCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATcc                       >  1:836679/1‑71 (MQ=255)
                               gCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATcc                       >  1:785657/1‑71 (MQ=255)
                               gCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATcc                       <  1:758721/71‑1 (MQ=255)
                                cTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaacaa                               <  1:1512703/62‑1 (MQ=255)
                                 tGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATccc                      >  1:1927114/1‑70 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCa                     <  1:1434777/69‑1 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                    >  1:1429881/1‑70 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                   >  1:674032/1‑71 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                   >  1:422543/1‑71 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                   >  1:271987/1‑71 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                   >  1:1432421/1‑71 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                   <  1:6005/71‑1 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                   <  1:1965137/71‑1 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                   >  1:1855398/1‑71 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                   <  1:1177473/71‑1 (MQ=255)
                                   atCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTAGGCCTTTAc                                          >  1:219900/2‑48 (MQ=11)
                                   atCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTAGGCCTTTAc                                          >  1:1685974/2‑48 (MQ=11)
                                    tCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCt                  >  1:1983398/1‑71 (MQ=255)
                                    tCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCt                  <  1:2102280/71‑1 (MQ=255)
                                      aTCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTgg                >  1:567323/1‑71 (MQ=255)
                                       tCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGtt                                            <  1:1976436/42‑1 (MQ=38)
                                       tCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGcc                                               <  1:1494913/39‑1 (MQ=255)
                                       tCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCt                            <  1:934836/58‑1 (MQ=255)
                                         ctctCAGACCAGCTAGGGATCTTCGCCTTGGTGAGCCg                                              >  1:2205879/1‑38 (MQ=255)
                                         ctctCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTA‑CCCACCAACAAGCTAATCCCATCTGGGca             >  1:1629522/1‑70 (MQ=255)
                                         ctctCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGca             >  1:669746/1‑71 (MQ=255)
                                            tcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATccc                      <  1:1727581/59‑1 (MQ=255)
                                            tcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATccc                      <  1:1665579/59‑1 (MQ=255)
                                            tcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACAt          >  1:2099376/1‑71 (MQ=255)
                                            tcAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCa                     <  1:1285282/60‑1 (MQ=255)
                                             cAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                   >  1:1802782/1‑61 (MQ=255)
                                             cAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATc         >  1:178734/1‑71 (MQ=255)
                                             cAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATc         >  1:1501515/1‑71 (MQ=255)
                                              aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTAc                                          >  1:614207/1‑37 (MQ=255)
                                              aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc        >  1:2070707/1‑71 (MQ=255)
                                              aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc        <  1:305451/71‑1 (MQ=255)
                                              aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc        <  1:70705/71‑1 (MQ=255)
                                              aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc        >  1:1555727/1‑71 (MQ=255)
                                               gACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                                   <  1:519351/43‑1 (MQ=255)
                                                aCCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGa      <  1:1387844/71‑1 (MQ=255)
                                                aCCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCt                            <  1:1875184/49‑1 (MQ=255)
                                                  cAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc        >  1:371481/1‑67 (MQ=255)
                                                   aGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATgg   <  1:2161217/71‑1 (MQ=255)
                                                    gCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAAt                         >  1:24950/1‑48 (MQ=255)
                                                    gCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                    >  1:508204/1‑53 (MQ=255)
                                                    gCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGca             <  1:70643/60‑1 (MQ=255)
                                                    gCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGc              >  1:790733/1‑59 (MQ=255)
                                                    gCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGc  <  1:736284/71‑1 (MQ=255)
                                                    gCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGc  <  1:1300605/71‑1 (MQ=255)
                                                      tAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGc  >  1:1520576/1‑69 (MQ=255)
                                                      |                                                                    
GGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGC  >  minE/1763502‑1763624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑