| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 204,556 | A→G | 100% | intergenic (+156/‑28) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 204,556 | 0 | A | G | 100.0% | 148.3 / NA | 52 | intergenic (+156/‑28) | alaV/rrlH | tRNA‑Ala/23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/29); total (23/29) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT > minE/204495‑204623 | ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:723006/71‑1 (MQ=255) cacTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATc > 1:139222/1‑71 (MQ=255) acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCCCAAGaaa < 1:1382796/61‑1 (MQ=11) caatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt < 1:91713/71‑1 (MQ=255) caatGAAAGGTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGt < 1:82659/71‑1 (MQ=11) tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa < 1:9204/56‑1 (MQ=255) tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:446841/2‑71 (MQ=19) tgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:2005859/2‑71 (MQ=19) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg > 1:1443480/1‑66 (MQ=25) gAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:1139096/1‑70 (MQ=25) aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACa > 1:445543/1‑57 (MQ=255) aaaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgt > 1:400503/1‑69 (MQ=25) ttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt < 1:1587779/71‑1 (MQ=21) tgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:110121/1‑70 (MQ=25) tgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:270392/1‑70 (MQ=25) tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc < 1:1337376/71‑1 (MQ=21) aGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt > 1:1151043/1‑70 (MQ=25) gTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt > 1:948690/1‑69 (MQ=25) gTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACt > 1:277234/1‑69 (MQ=25) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:1722115/38‑1 (MQ=255) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:1216065/38‑1 (MQ=255) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:2057977/38‑1 (MQ=255) tctctcAAATTTTCGCAACACGATGATGGATCGCAAGAAAc < 1:1628218/41‑1 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGaa < 1:362910/37‑1 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:415237/36‑1 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:821566/36‑1 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGtt < 1:756997/50‑1 (MQ=255) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:254933/1‑71 (MQ=21) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:851952/1‑71 (MQ=21) tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg > 1:436183/1‑71 (MQ=21) ctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTaa < 1:1966953/67‑1 (MQ=25) tcAAATTTTCGCAACACGATGATTGATCGCAAGaa < 1:222062/35‑1 (MQ=14) tcAAATTTTCGCAACACGATGATGGATGGCAAGAAACATCTTCGGGTtg > 1:1287880/1‑49 (MQ=11) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtg > 1:1363722/1‑49 (MQ=255) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:1426024/69‑1 (MQ=25) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:1187609/69‑1 (MQ=25) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:2185200/59‑1 (MQ=255) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:2028842/59‑1 (MQ=255) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:958941/1‑70 (MQ=25) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:99293/1‑70 (MQ=25) aaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTacac < 1:1571887/71‑1 (MQ=21) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:610284/70‑1 (MQ=25) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:834742/70‑1 (MQ=25) tCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:373976/70‑1 (MQ=25) cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTgg < 1:555312/70‑1 (MQ=25) cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTgg < 1:912219/70‑1 (MQ=25) cGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTgg < 1:177265/70‑1 (MQ=25) acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGc < 1:644763/55‑1 (MQ=255) acacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGcc > 1:1662207/1‑71 (MQ=21) cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCt > 1:1102897/1‑70 (MQ=25) cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTg > 1:874537/1‑71 (MQ=21) cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTg > 1:877001/1‑71 (MQ=21) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGt < 1:1586844/71‑1 (MQ=21) | TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT > minE/204495‑204623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |