Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,807,377 | T→C | 6.4% | intergenic (+58/+6) | emrB → / ← luxS | multidrug efflux system protein/S‑ribosylhomocysteinase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,807,377 | 0 | T | C | 6.4% | 160.5 / 4.0 | 62 | intergenic (+58/+6) | emrB/luxS | multidrug efflux system protein/S‑ribosylhomocysteinase |
Reads supporting (aligned to +/- strand): ref base T (27/31); new base C (2/2); total (29/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 |
CACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGAT > minE/1807314‑1807442 | cACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc > 1:878929/1‑70 (MQ=255) cACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc > 1:1406649/1‑70 (MQ=255) aCTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa > 1:681127/1‑64 (MQ=255) cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc > 1:1721617/1‑68 (MQ=255) aattACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGa < 1:238389/67‑1 (MQ=255) aGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGa < 1:1463810/69‑1 (MQ=255) gTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTa > 1:1524576/1‑59 (MQ=255) aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgtg > 1:278180/1‑70 (MQ=255) aCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtg > 1:318135/1‑68 (MQ=255) aaGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTAc > 1:99019/1‑52 (MQ=255) aaGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTcc > 1:670367/1‑71 (MQ=255) aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCa > 1:1251888/1‑65 (MQ=255) ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGc < 1:1347257/71‑1 (MQ=255) acAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGt < 1:1054118/64‑1 (MQ=255) gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAAc < 1:1314936/71‑1 (MQ=255) gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAAc < 1:1267563/71‑1 (MQ=255) tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGAc < 1:1361459/48‑1 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc > 1:1614294/1‑71 (MQ=255) cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtc > 1:2118426/1‑71 (MQ=255) aTTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGtt < 1:278143/59‑1 (MQ=255) tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGa > 1:2205516/1‑47 (MQ=255) tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGa > 1:663549/1‑47 (MQ=255) tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGAt > 1:599382/1‑48 (MQ=255) tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTctct < 1:48841/70‑1 (MQ=255) cTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTGTTTCgg < 1:1918863/71‑1 (MQ=255) cTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCgg < 1:1920989/71‑1 (MQ=255) cTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCgg < 1:859961/71‑1 (MQ=255) cTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCgg < 1:508361/71‑1 (MQ=255) tGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCgg > 1:568936/1‑70 (MQ=255) tGGCTTTTTTCAATTAATTGTGAAGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCttt < 1:914176/67‑1 (MQ=255) gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc < 1:817433/69‑1 (MQ=255) gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc < 1:1965675/69‑1 (MQ=255) gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCa > 1:189495/1‑70 (MQ=255) gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAg > 1:278501/1‑71 (MQ=255) ttttCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc > 1:1136501/1‑70 (MQ=255) ttttCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc > 1:1291037/1‑70 (MQ=255) tCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCtt > 1:443198/1‑57 (MQ=255) aattaattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCa < 1:364064/67‑1 (MQ=255) attaattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCtt < 1:1406361/54‑1 (MQ=255) attaattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCtt < 1:1580237/54‑1 (MQ=255) attaattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCtt < 1:179381/54‑1 (MQ=255) ttaattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCgg < 1:2117578/57‑1 (MQ=255) ttaattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttctt > 1:1145562/1‑71 (MQ=255) ttaattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttctt > 1:530812/1‑71 (MQ=255) aattGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttcttc < 1:411884/70‑1 (MQ=255) attGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttcttc < 1:1992819/69‑1 (MQ=255) attGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGt > 1:1632488/1‑71 (MQ=255) ttGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGtt < 1:1321306/71‑1 (MQ=255) gtgAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTg < 1:687084/70‑1 (MQ=255) aaGATAGTTCACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGt > 1:2186135/1‑71 (MQ=255) aGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAg > 1:903437/1‑50 (MQ=255) aGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt < 1:1849907/71‑1 (MQ=255) aGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt < 1:1226061/71‑1 (MQ=255) aGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt < 1:494710/71‑1 (MQ=255) aGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt < 1:1020474/71‑1 (MQ=255) aTAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCATTGCCAGTTCTTCGTTGCTGTTGa > 1:705634/1‑71 (MQ=255) aTAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttct < 1:1024157/58‑1 (MQ=255) aTAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt < 1:255325/69‑1 (MQ=255) tAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGa > 1:540122/1‑70 (MQ=255) tAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGa > 1:286497/1‑70 (MQ=255) tAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGAt < 1:227794/71‑1 (MQ=255) aGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt < 1:782413/67‑1 (MQ=255) ttACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGa > 1:43132/1‑66 (MQ=255) | CACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGAT > minE/1807314‑1807442 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |