| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,827,932 | C→T | 34.1% | A392V (GCG→GTG) | mutS → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,827,932 | 0 | C | T | 34.1% | 55.0 / 34.0 | 44 | A392V (GCG→GTG) | mutS | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base C (7/22); new base T (6/9); total (13/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAC > minE/1827862‑1828000 | cgcCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAG‑TAGAAACTGTCGATAGTGCACCGGTACAGgc > 1:973004/1‑70 (MQ=255) ccACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGgcgc < 1:1243710/71‑1 (MQ=255) tCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGt > 1:1182078/1‑68 (MQ=255) aCTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:2018227/66‑1 (MQ=255) gCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGc > 1:468670/1‑70 (MQ=255) gCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGc > 1:236606/1‑70 (MQ=255) gCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGc > 1:1564642/1‑70 (MQ=255) aGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGttt < 1:65188/71‑1 (MQ=255) gCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGtt < 1:1890282/69‑1 (MQ=255) gtgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAg < 1:1895208/71‑1 (MQ=255) tgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:982244/51‑1 (MQ=255) tgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:405504/51‑1 (MQ=255) tgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:2023933/51‑1 (MQ=255) tgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:1874658/51‑1 (MQ=255) tgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAg < 1:1216474/70‑1 (MQ=255) ttAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGc < 1:762782/51‑1 (MQ=255) ttAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGc < 1:208997/51‑1 (MQ=255) ttAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGa < 1:903191/71‑1 (MQ=255) ttAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGa < 1:1844260/71‑1 (MQ=255) ttAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGa < 1:899334/71‑1 (MQ=255) tAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGa < 1:1281861/70‑1 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGcc > 1:1572571/1‑58 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATc > 1:567845/1‑71 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATc > 1:1285113/1‑71 (MQ=255) aaaCTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGttt < 1:605073/53‑1 (MQ=255) aaaCTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGttt < 1:1981482/53‑1 (MQ=255) aaaCTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGttt < 1:1984084/53‑1 (MQ=255) aaaCTGTCGATAGTGCACCGGTACAGGCGATACGTGAGAAGATGGGCGAGttt < 1:1883308/53‑1 (MQ=255) gTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGa < 1:1500058/44‑1 (MQ=255) aTAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGcgcg < 1:1499537/57‑1 (MQ=255) aTAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagcga < 1:1921347/71‑1 (MQ=255) tAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagcgag < 1:2111588/71‑1 (MQ=255) tAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagcgag < 1:527631/71‑1 (MQ=255) aGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagcgag > 1:19658/1‑70 (MQ=255) tGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCaa < 1:1184355/71‑1 (MQ=255) cACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatc < 1:104347/71‑1 (MQ=255) cACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAat < 1:1413474/70‑1 (MQ=255) cACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAat < 1:1470820/70‑1 (MQ=255) cGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcat > 1:939503/1‑70 (MQ=255) gTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGa < 1:1394823/71‑1 (MQ=255) tACAGGCGCTACGTGAGAAGTTGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGac < 1:1814259/71‑1 (MQ=255) tACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGa > 1:1414088/1‑70 (MQ=255) aGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAat > 1:1233684/1‑61 (MQ=255) aGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAat > 1:878204/1‑61 (MQ=255) ggCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcatc < 1:380357/64‑1 (MQ=255) ggCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGacacac > 1:1996649/1‑71 (MQ=255) gcgcTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGaca > 1:1218949/1‑67 (MQ=255) | CGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGACACAC > minE/1827862‑1828000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |