| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,833,531 | C→T | 41.3% | R92C (CGC→TGC) | ygbL → | predicted class II aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,833,531 | 0 | C | T | 41.3% | 28.5 / 51.7 | 46 | R92C (CGC→TGC) | ygbL | predicted class II aldolase |
| Reads supporting (aligned to +/- strand): ref base C (14/13); new base T (11/8); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGA > minE/1833463‑1833598 | aaTGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCgtgc < 1:2143878/71‑1 (MQ=255) gTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTa < 1:1469001/71‑1 (MQ=255) aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtgg > 1:1999314/1‑69 (MQ=255) aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtgg > 1:1544047/1‑69 (MQ=255) aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtgg > 1:484380/1‑69 (MQ=255) aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtgg > 1:486984/1‑69 (MQ=255) aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg > 1:412958/1‑68 (MQ=255) aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg > 1:571993/1‑68 (MQ=255) cAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGCTGGTGc < 1:738210/71‑1 (MQ=255) aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCa > 1:926848/1‑71 (MQ=255) aCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAtt > 1:524186/1‑71 (MQ=255) ccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTg < 1:1026852/71‑1 (MQ=255) ccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTg < 1:441724/71‑1 (MQ=255) tCGAAAGATGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGc < 1:110350/64‑1 (MQ=255) gAAAGAGGTGCTCTTTCATCTGGCTCTGTATCGCAACAATCCGTGCTGTAAAGCggtgg > 1:1794293/1‑59 (MQ=255) gAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgca < 1:139737/69‑1 (MQ=255) gAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTgca > 1:1139802/1‑69 (MQ=255) tGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATgg < 1:1070975/71‑1 (MQ=255) ctTTCATCTGGCGCTGTATCGCAACAATCCTCGCTGTAAAGCGGTGGTGCATTTgcaca < 1:1204445/59‑1 (MQ=255) ctTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGa > 1:243732/1‑71 (MQ=255) tttCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCggt < 1:682128/44‑1 (MQ=255) tttCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCggt < 1:1855314/44‑1 (MQ=255) tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcacagcaca > 1:94713/1‑61 (MQ=255) tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcacagcaca > 1:639439/1‑61 (MQ=255) tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcacagcaca > 1:2182581/1‑61 (MQ=255) tCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCggtg > 1:2161693/1‑43 (MQ=255) tCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACgg > 1:171382/1‑71 (MQ=255) ggCGCTGTATCGCAACAATCCGCGCTGTAAAGCggtggt > 1:1834796/1‑39 (MQ=255) gcgcTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCtt < 1:1278915/69‑1 (MQ=255) tGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt < 1:2116278/66‑1 (MQ=255) tATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt > 1:550471/1‑64 (MQ=255) tCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:1474944/71‑1 (MQ=255) tCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:1088157/71‑1 (MQ=255) tCGCAACAATCCGCGCTGTAAAGCGGTTGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:1206888/71‑1 (MQ=255) tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctgcctg > 1:1481293/1‑70 (MQ=255) tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctgcctg > 1:881752/1‑70 (MQ=255) tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctg < 1:1603360/66‑1 (MQ=255) tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:302785/71‑1 (MQ=255) tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:1731938/71‑1 (MQ=255) tCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:1248207/71‑1 (MQ=255) gCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc < 1:503158/69‑1 (MQ=255) gCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc > 1:1030593/1‑69 (MQ=255) gCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc > 1:972241/1‑69 (MQ=255) gCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc > 1:125385/1‑69 (MQ=255) gaacaaTCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAg > 1:940765/2‑71 (MQ=255) acaaTCCTCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAggg < 1:136197/71‑1 (MQ=255) aaTCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt > 1:245978/1‑71 (MQ=255) cgcgCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGa > 1:978695/1‑70 (MQ=255) | AATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGA > minE/1833463‑1833598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |