| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,867,615 | G→A | 37.3% | T429I (ACC→ATC) | ygcU ← | predicted FAD containing dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,867,615 | 0 | G | A | 37.3% | 64.1 / 70.6 | 67 | T429I (ACC→ATC) | ygcU | predicted FAD containing dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (19/23); new base A (14/11); total (33/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTACAGTC > minE/1867552‑1867682 | cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTttcttc < 1:491824/71‑1 (MQ=255)cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt < 1:957227/70‑1 (MQ=255) cAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTca > 1:1118458/1‑69 (MQ=255) gTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTttctt > 1:1918189/1‑65 (MQ=255) gTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTttctt > 1:516821/1‑65 (MQ=255) gTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAg < 1:1428153/70‑1 (MQ=255) gTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAg < 1:1549281/70‑1 (MQ=255) gTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCCAATGGTttctt > 1:1231791/1‑65 (MQ=255) tGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTca < 1:1200003/66‑1 (MQ=255) gAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAgat > 1:955910/1‑70 (MQ=255) aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga > 1:369690/1‑71 (MQ=255) aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga > 1:445121/1‑71 (MQ=255) cgATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCtt < 1:697156/71‑1 (MQ=255) cgATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCtt < 1:948599/71‑1 (MQ=255) tGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCttgtt > 1:1521349/1‑71 (MQ=255) tGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttgtt > 1:779445/1‑71 (MQ=255) tGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttgtt > 1:431716/1‑71 (MQ=255) gTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTttc > 1:1915810/1‑52 (MQ=255) gTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTttc > 1:1670283/1‑52 (MQ=255) tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAga > 1:736781/1‑57 (MQ=255) tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGc > 1:77034/1‑71 (MQ=255) tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTATTCACAga > 1:579975/1‑57 (MQ=255) tACCGAGACCGTGGTGGTGCACCATCTAACCGCCGAGGCGAATGGTTTCTTCACAga > 1:2073/1‑57 (MQ=255) tACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATtg < 1:1084745/71‑1 (MQ=255) tACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtg < 1:751241/71‑1 (MQ=255) tggtggtgCACCATCGAACCGCCGAGGCGAATGAtt < 1:1897291/36‑1 (MQ=255) tggtgCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCt < 1:318249/49‑1 (MQ=255) tgCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTc < 1:1535203/71‑1 (MQ=255) tgCACCATCCAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTc < 1:831215/71‑1 (MQ=255) ccATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt > 1:1819259/1‑71 (MQ=255) ccATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt > 1:567540/1‑71 (MQ=255) ccATCGAACCGCCGAGGCGAAAGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt < 1:277575/71‑1 (MQ=255) cATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAttt < 1:1248316/71‑1 (MQ=255) cATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAttt < 1:2057033/71‑1 (MQ=255) cATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt < 1:1853991/70‑1 (MQ=255) aTCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCgg < 1:199399/50‑1 (MQ=255) aTCGAACCGCCGAGGCGAATGATTTCTTCAAAgatga > 1:300472/1‑37 (MQ=38) tCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTca < 1:1293160/71‑2 (MQ=255) cGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCct > 1:2153056/1‑71 (MQ=255) gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttg > 1:323393/1‑39 (MQ=255) gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttg > 1:20253/1‑39 (MQ=255) gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttg > 1:525936/1‑39 (MQ=255) gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAt > 1:297684/1‑49 (MQ=255) gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAt > 1:1179024/1‑49 (MQ=255) gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt > 1:2000133/1‑66 (MQ=255) gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCctc > 1:202500/1‑71 (MQ=255) gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCctc > 1:892461/1‑71 (MQ=255) aaCCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCctct < 1:755960/71‑1 (MQ=255) aaCCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCctct < 1:474154/71‑1 (MQ=255) gAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCt < 1:655070/71‑1 (MQ=255) aGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCtt < 1:1832866/71‑1 (MQ=255) aGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCtt < 1:194005/71‑1 (MQ=255) aGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt < 1:502917/57‑1 (MQ=255) ggCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCtt < 1:2128998/70‑1 (MQ=255) ggCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTa < 1:1684332/71‑1 (MQ=255) gCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTa > 1:1752670/1‑70 (MQ=255) cGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATtgtg < 1:2203937/41‑1 (MQ=255) cGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTc > 1:985683/1‑56 (MQ=255) aaTGGTTTCTTCACAGATGATCTTGTTGAGCGGATtgt < 1:628518/38‑1 (MQ=255) aaTGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg < 1:56447/46‑1 (MQ=255) aaTGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTACAGt < 1:1880458/71‑1 (MQ=255) aaTGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTACAGt < 1:1722814/71‑1 (MQ=255) aaTGATTTCTTCACAGATGATCTTGTTGAGCGGATtgt < 1:1950874/38‑1 (MQ=255) aTGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTACAGt > 1:735569/1‑70 (MQ=255) aTGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTACAGTc < 1:1069273/71‑1 (MQ=255) tGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTAc > 1:1868810/1‑41 (MQ=255) tGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTAGTAc > 1:153030/1‑41 (MQ=255) | CTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTACAGTC > minE/1867552‑1867682 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |