| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,874,329 | A→G | 14.1% | I191T (ATT→ACT) | ygcF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,874,329 | 0 | A | G | 14.1% | 217.2 / 28.9 | 92 | I191T (ATT→ACT) | ygcF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (45/34); new base G (7/6); total (52/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCA > minE/1874260‑1874397 | aTCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAAt > 1:323758/1‑71 (MQ=255) tCGACAAACGCGAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATc < 1:1608041/71‑1 (MQ=255) cGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCg > 1:2047372/1‑71 (MQ=255) gACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCgg > 1:769002/1‑71 (MQ=255) cAAACGCCAATTACGCGCAATGCAGGTTT‑AATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTg < 1:881480/71‑1 (MQ=255) aaaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTg > 1:1580084/1‑71 (MQ=255) aaaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTg < 1:988088/71‑1 (MQ=255) aaaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTg > 1:1812777/1‑71 (MQ=255) aaaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTg > 1:548926/1‑71 (MQ=255) aaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCt > 1:640291/1‑69 (MQ=255) aaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTg > 1:1945813/1‑70 (MQ=255) aaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGc < 1:1172703/71‑1 (MQ=255) aaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGc < 1:2203789/71‑1 (MQ=255) ccAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTg < 1:2070837/71‑1 (MQ=255) cAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGc < 1:1136775/71‑1 (MQ=255) ttACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAACCGGCTGCAGTGCAAt < 1:1443476/71‑1 (MQ=255) aCGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAAt > 1:1034232/1‑54 (MQ=255) aCGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATg < 1:1022664/70‑1 (MQ=255) aCGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGATGCAGTGCAAt < 1:363815/69‑1 (MQ=255) cgcgCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCaa < 1:848509/67‑1 (MQ=255) gcAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTaa > 1:1264026/1‑49 (MQ=255) gcAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTcg > 1:876365/1‑71 (MQ=255) gcAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTc > 1:2041377/1‑70 (MQ=255) gcAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTc > 1:252446/1‑70 (MQ=255) cAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTcgc < 1:780853/71‑1 (MQ=255) aaTGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTcgcg > 1:511683/1‑71 (MQ=255) tGCAGGTTTCAATGCACAAACGTGTGGCTTCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGt > 1:1392467/1‑71 (MQ=255) gCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGtt > 1:883866/1‑71 (MQ=255) gCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGtt > 1:650848/1‑71 (MQ=255) gCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGtt > 1:1726879/1‑71 (MQ=255) cAGGTTTCAATGCACTAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGtt > 1:809218/1‑70 (MQ=255) cAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGtt > 1:886078/1‑70 (MQ=255) cAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGtt > 1:185638/1‑70 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTg < 1:986935/47‑1 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatc < 1:311595/71‑1 (MQ=255) tttCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAAt < 1:715661/55‑1 (MQ=255) ttCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGAc > 1:1179463/1‑57 (MQ=255) tCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGAc > 1:2037286/1‑56 (MQ=255) tCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatcatc > 1:955250/1‑71 (MQ=255) tCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatcatc > 1:2111942/1‑71 (MQ=255) tCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatcatc > 1:1769327/1‑71 (MQ=255) tCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGAAATGACTCGCGGTTTatcatc > 1:1368774/1‑71 (MQ=255) cAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCg > 1:826872/1‑71 (MQ=255) aaTGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCgg > 1:280515/1‑71 (MQ=255) aaTGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCg > 1:1954765/1‑70 (MQ=255) tGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatcatc < 1:1325346/67‑1 (MQ=255) tGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatcatc < 1:990160/67‑1 (MQ=255) tGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatcat > 1:1135183/1‑66 (MQ=255) tGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatcat > 1:378564/1‑66 (MQ=255) tGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTatc < 1:1116464/64‑1 (MQ=255) tGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGt > 1:532782/1‑70 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTg > 1:326987/1‑43 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAg > 1:276837/1‑71 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTc > 1:130488/1‑52 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGt > 1:966400/1‑57 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGt > 1:1819316/1‑57 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCg > 1:80131/1‑66 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCg > 1:1679210/1‑66 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAg > 1:1481507/1‑71 (MQ=255) aaaCGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTat > 1:521212/1‑58 (MQ=255) aaCGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGtt > 1:989942/1‑71 (MQ=255) aaCGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGtt > 1:1511809/1‑71 (MQ=255) gtgtGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGcc < 1:248147/71‑1 (MQ=255) gtgtGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGcc < 1:1314592/71‑1 (MQ=255) gtgtGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGcc < 1:602157/71‑1 (MQ=255) gtgGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCag > 1:592173/1‑71 (MQ=255) tgGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagt < 1:1061552/71‑1 (MQ=255) tgGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagt < 1:1584708/71‑1 (MQ=255) tgGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCag < 1:1223095/70‑1 (MQ=255) tgGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCag > 1:1547261/1‑70 (MQ=255) tgGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCag < 1:356028/70‑1 (MQ=255) ggCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCag > 1:128984/1‑69 (MQ=255) tcatcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagt < 1:1316194/66‑1 (MQ=255) atcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCa > 1:314278/1‑70 (MQ=255) atcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCAt > 1:1624294/1‑71 (MQ=255) tcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATc < 1:797987/71‑1 (MQ=255) tcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATc < 1:228984/71‑1 (MQ=255) tcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATc < 1:614528/71‑1 (MQ=255) tcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATc < 1:504870/71‑1 (MQ=255) tcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTCCCAGTAGTTCATc < 1:47546/71‑1 (MQ=255) tcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGGCAGCGTTGCCAGTAGTTCATc < 1:853893/71‑1 (MQ=255) ccTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTat < 1:1845491/42‑1 (MQ=255) ccTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATc > 1:1964057/1‑70 (MQ=255) ccTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATc > 1:1562860/1‑70 (MQ=255) cTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATcc < 1:124716/70‑1 (MQ=255) cTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATcc < 1:502113/70‑1 (MQ=255) cTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATcc < 1:739417/70‑1 (MQ=255) ttttGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGc < 1:550623/51‑1 (MQ=255) ttGGCTAATCGGATGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagtag > 1:326755/1‑60 (MQ=255) cTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc < 1:2007573/71‑1 (MQ=255) cTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc < 1:1519919/71‑1 (MQ=255) cTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc < 1:992114/71‑1 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc > 1:1453954/1‑70 (MQ=255) aaTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCa > 1:1578003/1‑70 (MQ=255) | ATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCA > minE/1874260‑1874397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |