| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,883,198 | A→G | 22.5% | F429L (TTC→CTC) | rumA ← | 23S rRNA (uracil‑5)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,883,198 | 0 | A | G | 22.5% | 74.5 / 20.9 | 40 | F429L (TTC→CTC) | rumA | 23S rRNA (uracil‑5)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (13/18); new base G (6/3); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
AACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTA > minE/1883128‑1883265 | aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:192118/71‑1 (MQ=255)aaCAATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:1478940/71‑1 (MQ=255) ccATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa > 1:1587042/1‑69 (MQ=255) tCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCTAGAGAAg > 1:2167994/1‑66 (MQ=255) tCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAg > 1:1976482/1‑66 (MQ=255) ctctCCTTTAGGTACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATc < 1:1535057/71‑1 (MQ=255) ctctCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATc < 1:336605/71‑1 (MQ=255) ctctCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATc < 1:753933/71‑1 (MQ=255) ccTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaaaa > 1:832375/1‑59 (MQ=255) ccTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaaaa > 1:1323113/1‑59 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGa < 1:1442828/68‑1 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTc > 1:1886353/1‑71 (MQ=255) tttAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGt < 1:729129/59‑1 (MQ=255) ttAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:1926004/54‑1 (MQ=255) aGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCAta > 1:750280/1‑61 (MQ=255) aGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCAt > 1:929322/1‑61 (MQ=255) aGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATc > 1:1739600/1‑62 (MQ=255) aCCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:727212/48‑1 (MQ=255) aCCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:2051491/48‑1 (MQ=255) aCCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCa < 1:1095673/65‑1 (MQ=255) aCCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCa < 1:147322/65‑1 (MQ=255) cAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:1812781/46‑1 (MQ=255) cAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCa < 1:607400/63‑1 (MQ=255) cAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCa < 1:1287487/63‑1 (MQ=255) aGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGTAAAGTACCATCGATTc < 1:796254/60‑1 (MQ=255) ccTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg < 1:1660006/70‑1 (MQ=255) cTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCgtgtg > 1:1419669/1‑71 (MQ=255) cTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCgtgtg > 1:1930120/1‑71 (MQ=255) cGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGa > 1:1731058/1‑56 (MQ=255) gAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtgtg > 1:661644/1‑66 (MQ=255) gAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTggg > 1:1292422/1‑70 (MQ=255) aaTCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAc < 1:1213410/40‑1 (MQ=255) aTCGGCAAATCGCAACTATTTAACGCGCGAGaaaa > 1:1556475/1‑35 (MQ=255) aaTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTc < 1:1784565/41‑1 (MQ=255) tCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCa < 1:120541/41‑1 (MQ=255) tCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCa < 1:2176973/41‑1 (MQ=255) cGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCAt > 1:1811235/1‑71 (MQ=255) aaCTATTTAACGCGCGAGAAAAGTACCATCGATTCCa < 1:1281355/37‑1 (MQ=255) aCTATTTAACGCGCGAGAAAAGAACCATCGATTCCAGATGTCCCgtgtgt > 1:325842/1‑50 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGc > 1:1867361/1‑62 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt > 1:370666/1‑71 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt > 1:1447195/1‑71 (MQ=255) tAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgc > 1:2120895/1‑71 (MQ=255) tAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgc > 1:1188574/1‑71 (MQ=255) aCGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCgcgc < 1:2106602/71‑1 (MQ=255) aCGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCgcgc < 1:106860/71‑1 (MQ=255) cgcgAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAAt < 1:101776/71‑1 (MQ=255) gcgAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCTCGCAATg < 1:148450/71‑1 (MQ=255) gagaAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGt < 1:1929028/71‑1 (MQ=255) gaAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGta > 1:20072/1‑70 (MQ=255) | AACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTA > minE/1883128‑1883265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |