| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,890,961 | G→A | 14.3% | P208S (CCG→TCG) | gudP ← | predicted D‑glucarate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,890,961 | 0 | G | A | 14.3% | 90.5 / 7.4 | 35 | P208S (CCG→TCG) | gudP | predicted D‑glucarate transporter |
| Reads supporting (aligned to +/- strand): ref base G (20/10); new base A (2/3); total (22/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
CTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAG > minE/1890896‑1891031 | cTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGtt > 1:670733/1‑71 (MQ=255)cTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCAGGATGtt < 1:603133/71‑1 (MQ=255) tGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGAt > 1:1283002/1‑67 (MQ=255) aTCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGa < 1:946144/70‑1 (MQ=255) tatTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGAt < 1:1983353/66‑1 (MQ=255) ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTg > 1:1930399/1‑67 (MQ=255) ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCt > 1:483951/1‑70 (MQ=255) aTCAGCGCACCACCCGCGGCGATGTACTTCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCt < 1:1802614/67‑1 (MQ=255) aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCt < 1:1590880/67‑1 (MQ=255) aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTg > 1:258152/1‑71 (MQ=255) aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTg < 1:2188296/71‑1 (MQ=255) tCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTgg < 1:51996/71‑1 (MQ=255) cAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGAt > 1:864411/1‑59 (MQ=255) cAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTc > 1:1401316/1‑66 (MQ=255) cAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGa > 1:257454/1‑71 (MQ=255) cAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACACCCGGATGTTGAt > 1:1051786/1‑59 (MQ=255) aGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGAt < 1:214041/71‑1 (MQ=255) aGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGAt < 1:923021/71‑1 (MQ=255) aGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTAGTGGAt < 1:4189/71‑1 (MQ=255) accCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTc > 1:1658827/1‑70 (MQ=255) gcggcgATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAAc > 1:346490/1‑69 (MQ=255) gcgATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGa > 1:506597/1‑70 (MQ=255) tACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt < 1:1858650/71‑1 (MQ=255) cTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGAc < 1:2085174/51‑1 (MQ=255) cAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCtgatga > 1:1605921/1‑71 (MQ=255) aGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCtgatga > 1:1020219/1‑70 (MQ=255) ctctTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGAc < 1:825928/45‑1 (MQ=255) ctctTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGAGTTTCaa > 1:1830379/1‑51 (MQ=255) tctttcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAAt > 1:1742090/1‑71 (MQ=255) ctttcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACttt > 1:575220/1‑46 (MQ=255) ctttcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATc > 1:1101378/1‑71 (MQ=255) ctttcttATTTACCCCCGGATGTTGAATTGGCTCGTGGATGACTTTCAAACAGATAAAGCTGATGACAATc > 1:17344/1‑71 (MQ=255) tcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATgg > 1:2168546/1‑38 (MQ=255) tcttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACaa > 1:302990/1‑66 (MQ=255) tACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAAcc > 1:38343/1‑41 (MQ=255) gATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAaag < 1:1166216/71‑1 (MQ=255) gATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAaag < 1:771309/71‑1 (MQ=255) | CTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGACCGCCCATAAAG > minE/1890896‑1891031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |