| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,144,345 | G→A | 13.3% | D206N (GAT→AAT) | argG → | argininosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,144,345 | 0 | G | A | 13.3% | 118.5 / 9.1 | 45 | D206N (GAT→AAT) | argG | argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (17/21); new base A (3/3); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAT > minE/2144275‑2144410 | cTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCTAAgg < 1:1582816/71‑1 (MQ=255) aaTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTg > 1:1546749/1‑70 (MQ=255) aTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGAt > 1:944713/1‑66 (MQ=255) tcGAAAAAGCCTACTCCACAGCCTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTc < 1:2184836/71‑1 (MQ=255) tcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:289380/63‑1 (MQ=255) aaGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTcc < 1:1648376/71‑1 (MQ=255) cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGaa > 1:1228614/1‑49 (MQ=255) cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGaa > 1:618925/1‑49 (MQ=255) cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:949909/1‑71 (MQ=255) cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaa < 1:1091290/70‑1 (MQ=255) ccAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTc < 1:2185299/47‑1 (MQ=255) cAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCa > 1:1424315/1‑53 (MQ=255) cAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCa > 1:1453132/1‑53 (MQ=255) cAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTc > 1:280699/1‑67 (MQ=255) aaCATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAccc > 1:498122/1‑71 (MQ=255) aaCATGCTTGGTGCAACGCATGAAGCGAAGGATCAGGAATAc > 1:1287977/1‑42 (MQ=255) aCATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAccc > 1:742278/1‑70 (MQ=255) cATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGa > 1:1097019/1‑71 (MQ=255) aTGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:2125537/71‑1 (MQ=255) aTGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt > 1:752234/1‑71 (MQ=255) aTGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:253271/71‑1 (MQ=255) tGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAtt < 1:794976/71‑1 (MQ=255) gCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa > 1:616734/1‑71 (MQ=255) cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa < 1:1621110/70‑1 (MQ=255) cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa < 1:723416/70‑1 (MQ=255) cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa < 1:1099505/70‑1 (MQ=255) cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa < 1:52543/70‑1 (MQ=255) cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt > 1:2138256/1‑71 (MQ=255) gCAACGCATGAAGCGAAGTATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGt > 1:754317/1‑71 (MQ=255) cAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGGGTCAAAATCGTCAACCCGATTATGGGCGt > 1:2080030/1‑70 (MQ=255) aaCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGa < 1:2180675/71‑1 (MQ=255) aaCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGa < 1:1383694/71‑1 (MQ=255) aCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGa < 1:1014810/70‑1 (MQ=255) aCGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGa > 1:567252/1‑70 (MQ=255) cGCATTAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaa < 1:1066384/44‑1 (MQ=255) cGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaa < 1:2211165/44‑1 (MQ=255) cGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGaaa > 1:1241471/1‑71 (MQ=255) cATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:767502/71‑1 (MQ=255) aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGTGCGTGAAATTCTgg > 1:1728584/1‑71 (MQ=255) aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg > 1:635623/1‑71 (MQ=255) aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg < 1:2133270/71‑1 (MQ=255) aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg < 1:1057634/71‑1 (MQ=255) aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTg < 1:482547/70‑1 (MQ=255) cGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAt < 1:1054398/71‑1 (MQ=255) aaGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg < 1:726541/66‑1 (MQ=255) aaGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg < 1:98755/66‑1 (MQ=255) ggATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCt > 1:968904/1‑62 (MQ=255) | CTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAT > minE/2144275‑2144410 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |