| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,211,683 | A→G | 18.6% | I465I (ATT→ATC) | rng ← | ribonuclease G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,211,683 | 0 | A | G | 18.6% | 91.8 / 17.3 | 43 | I465I (ATT→ATC) | rng | ribonuclease G |
| Reads supporting (aligned to +/- strand): ref base A (20/15); new base G (4/4); total (24/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCATA > minE/2211613‑2211751 | tcatTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaa < 1:404652/71‑1 (MQ=255)tcatTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaa < 1:450382/71‑1 (MQ=255)tcatTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaa < 1:1351598/71‑1 (MQ=255) catTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaaa > 1:517866/1‑71 (MQ=255) atTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTGCTTTAACCTGTTTGCCAACGAAAAt > 1:115778/1‑71 (MQ=255) atTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAAt > 1:1462552/1‑71 (MQ=255) aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCAc > 1:197548/1‑66 (MQ=255) aaaCTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTcc > 1:1247890/1‑70 (MQ=255) cTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTc > 1:1214750/1‑60 (MQ=255) cTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaa < 1:91193/49‑1 (MQ=255) cTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgt > 1:1183196/1‑71 (MQ=255) tGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAgtg > 1:356843/1‑71 (MQ=255) ggTTATAGAGCGGTTCAATTTTTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt > 1:115365/1‑71 (MQ=255) gTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAgtgt < 1:1535818/70‑1 (MQ=255) gTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGtgtg < 1:1443985/71‑1 (MQ=255) gTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt < 1:833834/70‑1 (MQ=255) ttATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAGAtt < 1:1027135/48‑1 (MQ=255) ataGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGaaaa > 1:1391474/1‑44 (MQ=255) ataGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCa > 1:919867/1‑59 (MQ=255) agagCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCAc > 1:1377906/1‑49 (MQ=255) gagCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCtt < 1:867178/71‑1 (MQ=255) tCAATTTGTACTTTAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAGTGTGACACTTCGCCttt > 1:1186360/1‑71 (MQ=255) cAATTTGTACTTTAACCTGTTTGCCAACGAAAAttt > 1:1423090/1‑36 (MQ=255) aaTTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTc < 1:835020/70‑1 (MQ=255) aTTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGa > 1:648828/1‑50 (MQ=255) tttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTcc < 1:1935384/41‑1 (MQ=255) tttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt < 1:1161618/53‑1 (MQ=255) ttGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAgg > 1:1329379/1‑71 (MQ=255) tttAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGac < 1:1835361/43‑2 (MQ=255) tAACCTGTTTGCCAACGAAGATTTCCACTTCCGCCAGCGAgtg > 1:973805/1‑43 (MQ=255) tAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtg > 1:1557005/1‑43 (MQ=255) aCCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGc < 1:2037415/68‑1 (MQ=255) aCCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTAc > 1:161247/1‑71 (MQ=255) cTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt < 1:923377/40‑1 (MQ=255) cTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAgtgt < 1:2016227/40‑1 (MQ=255) gTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGa > 1:2133015/1‑40 (MQ=255) gTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGa > 1:457207/1‑40 (MQ=255) tttGCCAACGAAAATTTCCACTTCCGCCAGCGAGtgtg < 1:1994825/38‑1 (MQ=255) tttGCCAACGAAAATTTCCACTTCCGCCAGCGAGtgtg < 1:78434/38‑1 (MQ=255) tttGCCAACGAAAATTTCCACTTCCGCCAGCGAGtgtg < 1:1217101/38‑1 (MQ=255) tttGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCg < 1:741098/71‑1 (MQ=255) gCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGga < 1:1687427/70‑1 (MQ=255) ccAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGga < 1:2021039/69‑1 (MQ=255) aaCGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAg > 1:2054573/1‑71 (MQ=255) aaCGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAg > 1:1251650/1‑71 (MQ=255) aCGAAGATTTCCACTTCAGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGc < 1:1949891/71‑1 (MQ=255) gAAGATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCa > 1:1394016/1‑70 (MQ=255) gAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCa > 1:123711/1‑70 (MQ=255) gAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCa > 1:1678930/1‑70 (MQ=255) aaaaTTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCATa < 1:681289/71‑1 (MQ=255) | TCATTACGACGTCAAACTGCTCCTGGTTATAGAGCGGTTCAATTTGTACTTTAACCTGTTTGCCAACGAAAATTTCCACTTCCGCCAGCGAGTGTGACTCTTCGCCTTTCAAGGCTTCAGCTACTGCCGGAGAAGCATA > minE/2211613‑2211751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |