Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	656,039	Δ1 bp	100%	coding (125/2601 nt)	ycbS →	predicted outer membrane usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	656,039	0	C	.	100.0%	16.7 / NA	5	coding (125/2601 nt)	ycbS	predicted outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (5/0);  total (5/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCC  >  minE/656000‑656070                                        |                                cAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTG‑TTTTTTCAAAGATGATCCCTCa           >  1:482319/1‑61 (MQ=255) cAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTG‑TTTTTTCAAAGATGATCCCTCAATGGTGGcc  >  1:127500/1‑70 (MQ=255) cAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTG‑TTTTTTCAAAGATGATCCCTCAATGGTGGcc  >  1:189873/1‑70 (MQ=255) cAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTG‑TTTTTTCAAAGATGATCCCTCAATGGTGGcc  >  1:396754/1‑70 (MQ=255) cAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTG‑TTTTTTCAAAGATGATCCCTCAATGGTGGcc  >  1:582370/1‑70 (MQ=255)                                        |                                CAGCGTTCAATGCACAAGCTGAAACCTGGTTCGATCCTGCTTTTTTCAAAGATGATCCCTCAATGGTGGCC  >  minE/656000‑656070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑