Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 683,918 Δ1 bp 100% coding (555/663 nt) yccT ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE683,9180C.100.0% 54.8 / NA 13coding (555/663 nt)yccTconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (13/0);  total (13/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTG  >  minE/683899‑683970
                   |                                                    
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCt   >  1:237768/1‑70 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:139495/1‑71 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:169181/1‑71 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:206223/1‑71 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:243750/1‑71 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:253189/1‑71 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:380264/1‑71 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:482647/1‑71 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:490399/1‑71 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:497365/1‑71 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:531468/1‑71 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:534241/1‑71 (MQ=255)
cTGTGAGCACCTGAGACTGGGTGGAATAGCATCAAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg  >  1:543064/1‑71 (MQ=255)
                   |                                                    
CTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTG  >  minE/683899‑683970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: