| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,827,840 | Δ1 bp | 100% | coding (1083/2562 nt) | mutS → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,827,840 | 0 | C | . | 100.0% | 40.2 / NA | 10 | coding (1083/2562 nt) | mutS | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (0/10); total (0/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGC > minE/1827806‑1827877 | gTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc < 1:125644/71‑1 (MQ=255)gTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc < 1:12678/71‑1 (MQ=255)gTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc < 1:194779/71‑1 (MQ=255)gTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc < 1:450152/71‑1 (MQ=255)gTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc < 1:553907/71‑1 (MQ=255)gTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc < 1:60580/71‑1 (MQ=255)gTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc < 1:654887/71‑1 (MQ=255)gTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGACCCGTATGCGCCACGCTTTCCAGc < 1:297894/71‑1 (MQ=255) tATTCTGGCACGTCTGGCCTTACGAACTGCTCG‑CCACGCTATCTGGCCCGTATGCGCCACGCTTTCCAGc < 1:235027/70‑1 (MQ=255) gTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc < 1:14387/59‑1 (MQ=255) | GTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGC > minE/1827806‑1827877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |