| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 681,095:1 | +T | 100% | intergenic (‑48/‑75) | yccF ← / → helD | conserved inner membrane protein/DNA helicase IV |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 681,095 | 1 | . | T | 100.0% | 68.5 / NA | 19 | intergenic (‑48/‑75) | yccF/helD | conserved inner membrane protein/DNA helicase IV |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (19/0); total (19/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACG‑TTTTTTTGGGCTGGAGCACGTTTTCTCTGACGG > minE/681059‑681128 | ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:395372/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:658391/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:613332/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:568080/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:491215/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:452474/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:448426/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:10033/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:366200/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:308849/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:294682/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:238916/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:218468/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:205586/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:159724/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:122393/1‑71 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACg > 1:532884/1‑70 (MQ=255)ttACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACg > 1:643755/1‑70 (MQ=255)ttACTGTGGTTTTCAGTAATCGGCGGCAATTGTAACGTTTTTTTTGGGCTGGAGCACGTTTTCTCTGACgg > 1:562626/1‑71 (MQ=255) | TTACTGTGGTTTTCAGTAATGGGCGGCAATTGTAACG‑TTTTTTTGGGCTGGAGCACGTTTTCTCTGACGG > minE/681059‑681128 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |