| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,302,407:1 | +T | 100% | coding (674/1167 nt) | dacD ← | D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,302,407 | 1 | . | T | 100.0% | 34.7 / NA | 13 | T225N (ACC→AAC) | dacD | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (0/13); total (0/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACATTCATGG‑TTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTT > minE/1302397‑1302466 | aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:103346/71‑1 (MQ=255)aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:129425/71‑1 (MQ=255)aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:357296/71‑1 (MQ=255)aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:381785/71‑1 (MQ=255)aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:420861/71‑1 (MQ=255)aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:44034/71‑1 (MQ=255)aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:520612/71‑1 (MQ=255)aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:524366/71‑1 (MQ=255)aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:551279/71‑1 (MQ=255)aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:554080/71‑1 (MQ=255)aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:603537/71‑1 (MQ=255)aaCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:628173/71‑1 (MQ=255) aCATTCATGGTTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACtt < 1:615713/70‑1 (MQ=255) | AACATTCATGG‑TTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTT > minE/1302397‑1302466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |