| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,315,996:1 | +T | 100% | coding (594/738 nt) | hisA → | N‑(5'‑phospho‑L‑ribosyl‑formimino)‑5‑amino‑1‑ (5'‑phosphoribosyl)‑4‑imidazolecarboxamide isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,315,996 | 1 | . | T | 90.9% | 32.7 / ‑2.1 | 11 | coding (594/738 nt) | hisA | N‑(5'‑phospho‑L‑ribosyl‑formimino)‑5‑amino‑1‑ (5'‑phosphoribosyl)‑4‑imidazolecarboxamide isomerase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (10/0); total (11/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
AGGCTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCA‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCG > minE/1315942‑1316066 | aGGCTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCA‑TTTCAGTCCTCcggcg > 1:401062/1‑71 (MQ=255) tAACGTCTCTTTATATGAAGAAGTGTGTGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAt > 1:574210/1‑70 (MQ=255) tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:319481/1‑71 (MQ=255) tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:125975/1‑71 (MQ=255) tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:395163/1‑71 (MQ=255) tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:390433/1‑71 (MQ=255) tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:627017/1‑71 (MQ=255) tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:372090/1‑71 (MQ=255) tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAtt > 1:370744/1‑71 (MQ=255) tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTTCAGTCCTCCGGCGGTAt > 1:372834/1‑70 (MQ=255) tAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGAGGCATTTTCAGTCCTCCGGCGGTAtt > 1:253659/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGTACTGGTGTGCgcggcg > 1:643664/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGgt > 1:188727/1‑61 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:449407/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:451814/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:574252/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:462263/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:474971/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:478491/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:441760/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:505788/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:517025/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:527534/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:538439/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:540514/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:427963/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:542022/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:426045/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:644760/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:106612/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:91179/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:87494/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:85157/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:82864/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:82730/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:75622/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:68256/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:653875/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:646615/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:555216/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:641558/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:637835/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:634550/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:611825/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:59485/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:5834/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:579731/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:97178/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:568786/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:238449/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:298848/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:425175/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:295624/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:295569/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:286513/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:285455/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:283357/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:259664/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:301944/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:212601/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:212092/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:184587/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:183570/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:181157/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:178965/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:169304/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:159604/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:348068/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:117810/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:398230/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:386090/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:362439/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:361982/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:357717/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:142911/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:351613/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:350668/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:347370/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:346755/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:344438/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:335953/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:335617/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:330110/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:308933/1‑71 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggc > 1:297875/1‑70 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggc > 1:158255/1‑70 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggc > 1:291876/1‑70 (MQ=255) a‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGAGGCGGCCCTGCGTGGCACTGGTGTGCgcggc > 1:406675/1‑70 (MQ=255) a‑TTTCAGTCATCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCgcggcg > 1:470238/1‑71 (MQ=255) | AGGCTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCA‑TTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCG > minE/1315942‑1316066 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |