Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 72,873:1 +A 100% coding (106/1005 nt) fruR → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE72,8731.A96.7% 103.6 / ‑2.4 30coding (106/1005 nt)fruRDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (29/0);  total (30/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

CCGTGTGAGCGACAAAACCGTTG‑A‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTA  >  minE/72851‑72907
                       |                                   
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCtgtg                   >  1:372615/1‑41 (MQ=38)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACaa     >  1:613646/1‑55 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:42820/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:91461/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:653449/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:652782/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:644934/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:624708/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:566004/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:554402/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:524804/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:512104/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:484909/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:474093/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:450382/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:110265/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:411482/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:380695/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:371630/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:33500/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:334535/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:314416/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:314144/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:278750/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:246000/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:204287/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:160111/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:142240/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTGAA‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:129443/1‑58 (MQ=255)
ccGTGTGAGCGACAAAACCGTTG‑AGAAAAGTCATGGCTGTGGTGCGTGAGCACAATTa  >  1:458621/1‑58 (MQ=255)
                       |                                   
CCGTGTGAGCGACAAAACCGTTG‑A‑AAAAGTCATGGCTGTGGTGCGTGAGCACAATTA  >  minE/72851‑72907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑