| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 276,008:1 | +AC | 100% | coding (167/891 nt) | cyoE ← | protoheme IX farnesyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 276,008 | 1 | . | A | 94.7% | 131.3 / 1.3 | 38 | coding (167/891 nt) | cyoE | protoheme IX farnesyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (36/0); total (38/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
| * | minE | 276,008 | 2 | . | C | 94.7% | 136.4 / 0.6 | 38 | coding (167/891 nt) | cyoE | protoheme IX farnesyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (36/0); total (38/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAA‑‑ACACACAACCCGACGCCA > minE/275958‑276026 || tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGcc > 1:450852/1‑70 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:480081/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:113609/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:371811/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:398531/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:426453/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:444687/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:45819/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:47926/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:345929/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:506895/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:510401/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:533283/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:551668/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:611147/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:6671/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:81428/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:245324/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:133261/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:140756/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:132305/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:160266/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:191295/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:197209/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:218571/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:231214/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:339701/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:261131/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:281176/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:296597/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:323125/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:32627/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:125225/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAA‑‑Acacacacaa > 1:410028/1‑58 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAA‑‑Acacaca > 1:378590/1‑58 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGAAGTAGTTGTTAAACACACACAACCCGACGCca > 1:454279/1‑71 (MQ=255)tcttcGTCCTTTCCATCTTTCTGTCGATATCCCTGTAGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:149779/1‑71 (MQ=255)tcttcGGCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACACAACCCGACGCca > 1:343022/1‑71 (MQ=255) || TCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAA‑‑ACACACAACCCGACGCCA > minE/275958‑276026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |