| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,940,218:1 | +T | 100% | coding (121/267 nt) | ygfY ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,940,218 | 1 | . | T | 100.0% | 44.5 / NA | 14 | R41S (CGC→AGC) | ygfY | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (0/14); total (0/14) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCG‑TTTTTCGTCATCGCTTAAGCTGTCGTACTC > minE/1940179‑1940248 | ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:177842/71‑1 (MQ=255)ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:227434/71‑1 (MQ=255)ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:255933/71‑1 (MQ=255)ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:453722/71‑1 (MQ=255)ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:47354/71‑1 (MQ=255)ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:48124/71‑1 (MQ=255)ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:495426/71‑1 (MQ=255)ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:499217/71‑1 (MQ=255)ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:508526/71‑1 (MQ=255)ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:581229/71‑1 (MQ=255)ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:584960/71‑1 (MQ=255)ggTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:640536/71‑1 (MQ=255) tCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:107169/69‑1 (MQ=255) gcagACGAATAAAGATGCGTTTTTTCGTCATCGCTTAAGCTGTCGTACTc < 1:38577/50‑1 (MQ=255) | GGTCCGGATCGTCACATTCCAGCAGACGAATAAAGATGCG‑TTTTTCGTCATCGCTTAAGCTGTCGTACTC > minE/1940179‑1940248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |