| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,987,439:1 | +T | 100% | coding (284/1047 nt) | ansB ← | periplasmic L‑asparaginase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,987,439 | 1 | . | T | 83.9% | 89.3 / 14.3 | 31 | coding (284/1047 nt) | ansB | periplasmic L‑asparaginase II |
| Reads supporting (aligned to +/- strand): ref base . (0/5); new base T (0/26); total (0/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
TGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAA‑TTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGA > minE/1987397‑1987490 | tGGGTAATGACGAAGCCGTCGGTCTTGTCGCAGTCGGTGTTAA‑TTTTTT‑TCGCCAGTGTCAGCCAGACAtt < 1:101203/71‑1 (MQ=255)tGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAA‑TTTTTT‑TCGCCAGTGTCAGCCAGACAtt < 1:38505/71‑1 (MQ=255)tGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAA‑TTTTTT‑TCGCCAGTGTCAGCCAGACAtt < 1:351237/71‑1 (MQ=255)cgggTAATGACGAAGCCGTCGGGCTTATCGCAGTCGGTGTTAA‑TTTTTT‑TCGCCAGTGTCAGCCAGACAtt < 1:472354/70‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:3472/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:95348/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:547811/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:545077/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:514088/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:436159/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:431391/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:423630/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:370433/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:357605/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:349590/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:347734/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:300819/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:290000/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:289812/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:287774/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:279957/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:269129/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:261656/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:190428/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:181491/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:178533/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:154959/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:150576/69‑1 (MQ=255) aTCGCAGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:112306/69‑1 (MQ=255) aTCGCAGTCGGTGTTAA‑TTTTTTCTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:627058/69‑1 (MQ=255) aGTCGGTGTTAATTTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGa < 1:236975/64‑1 (MQ=255) | TGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAA‑TTTTTT‑TCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGA > minE/1987397‑1987490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |