| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 945 | A→G | 100% | G203G (GGA→GGG) | thrA → | fused aspartokinase I and homoserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 945 | 0 | A | G | 96.2% | 88.4 / ‑3.5 | 26 | G203G (GGA→GGG) | thrA | fused aspartokinase I and homoserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (0/25); total (0/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTC > minE/893‑965 | tGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTcc < 1:436443/65‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCTACTActc < 1:566179/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:5078/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:85994/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:7845/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:7025/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:646390/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:57410/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:572385/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:563268/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:550361/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:546445/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:513700/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:142191/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:402738/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:342740/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:34046/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:31789/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:2623/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:147108/71‑1 (MQ=255) aTGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:146339/71‑1 (MQ=255) ggCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:563498/69‑1 (MQ=255) ttCACCGCCGGTAATGAAAAAGGCGAACTGGTGGGGCTTGGGCGCAACGGTTCCGACTActc < 1:27857/62‑1 (MQ=255) aCCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:38248/59‑1 (MQ=255) tgTAATGAAAAAGGCGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:399151/52‑1 (MQ=38) cGAACTGGTGGTGCTTGGGCGCAACGGTTCCGACTActc < 1:205134/39‑1 (MQ=255) | TGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTC > minE/893‑965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |