| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 780,135 | T→C | 100% | Y81H (TAT→CAT) | ycgL → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 780,135 | 0 | T | C | 100.0% | 56.5 / NA | 16 | Y81H (TAT→CAT) | ycgL | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/0); total (16/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAG > minE/780128‑780197 | aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCa > 1:504814/1‑69 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:224453/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:230613/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:248284/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:339074/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:396136/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:438041/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:43846/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:49023/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:537485/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:571019/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:580955/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:598477/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:610621/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:8448/1‑70 (MQ=255)aGGTTACCATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAg > 1:98545/1‑70 (MQ=255) | AGGTTACTATTTGCAGTTACCGCCACCACCCGAAGATTTGCTGAAGCAACATCTTTCCGTCATGGGGCAG > minE/780128‑780197 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |