| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 825,185 | C→T | 100% | E32K (GAG→AAG) | ychN ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 825,185 | 0 | C | T | 100.0% | 61.8 / NA | 18 | E32K (GAG→AAG) | ychN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/18); total (0/18) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTCTCCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTGCGC > minE/825181‑825250 | ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:359329/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:70830/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:651022/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:630877/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:550986/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:545064/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:475391/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:470030/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:455380/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:36599/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:175200/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:346113/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:338908/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:324790/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:313475/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:294383/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:253713/70‑1 (MQ=255)ctctTCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:208587/70‑1 (MQ=255) tcttctGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTgcgc < 1:281150/69‑1 (MQ=255) | CTCTCCTGCTCTCGTAACGCAATGGCCAGCCGCAAGCTGTTAAACAAGGATTCGCTCCCGTAAGGTGCGC > minE/825181‑825250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |