Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	935,805	G→A	100%	R81H (CGC→CAC)	fdnH →	formate dehydrogenase‑N, Fe‑S (beta) subunit, nitrate‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	935,805	0	G	A	100.0%	16.7 / NA	6	R81H (CGC→CAC)	fdnH	formate dehydrogenase‑N, Fe‑S (beta) subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/6);  total (0/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTA  >  minE/935792‑935864              |                                                            gggACGGTGATGCACTTTAGCGAAACCGAACAGAACGGCAAGCGGGAGTGGCTGATCCGTAAAGACGGCTg    <  1:137903/70‑1 (MQ=38)   gACGGTGATGCACTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTg    <  1:197613/69‑1 (MQ=255)   gACGGTGATGCACTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTg    <  1:215612/69‑1 (MQ=255)   gACGGTGATGCACTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTg    <  1:437021/69‑1 (MQ=255)   gACGGTGATGCACTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTg    <  1:437421/69‑1 (MQ=255)   gACGGTGATGCACTTTAGCGAAACCGAACAGAACGGCAAGCGGGAGTGGCTGATCCGTAAAGACGGCTg    <  1:228566/69‑1 (MQ=38)            gcgcTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTa  >  1:74649/1‑62 (MQ=255)            gcgcTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTa  >  1:633300/1‑62 (MQ=255)            gcgcTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTa  >  1:610510/1‑62 (MQ=255)            gcgcTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTa  >  1:547089/1‑62 (MQ=255)            gcgcTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTa  >  1:541130/1‑62 (MQ=255)            gcgcTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTa  >  1:495167/1‑62 (MQ=255)            gcgcTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTa  >  1:47020/1‑62 (MQ=255)            gcgcTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTa  >  1:411992/1‑62 (MQ=255)            gcgcTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTa  >  1:258728/1‑62 (MQ=255)            gcgcTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTa  >  1:197552/1‑62 (MQ=255)            acgcTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTa  >  1:425203/2‑62 (MQ=255)              |                                                            TGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGTA  >  minE/935792‑935864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑