| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 979,595 | C→T | 100% | T105T (ACC→ACT) | ydfG → | L‑allo‑threonine dehydrogenase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 979,595 | 0 | C | T | 100.0% | 32.8 / NA | 10 | T105T (ACC→ACT) | ydfG | L‑allo‑threonine dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/0); total (10/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGC > minE/979556‑979627 | tAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACTAACAACAAAGGCCTGGTATATATGAcgcgcg > 1:229576/1‑71 (MQ=255)tAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACTAACAACAAAGGCCTGGTATATATGAcgcgcg > 1:264749/1‑71 (MQ=255)tAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACTAACAACAAAGGCCTGGTATATATGAcgcgcg > 1:303070/1‑71 (MQ=255)tAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACTAACAACAAAGGCCTGGTATATATGAcgcgcg > 1:356958/1‑71 (MQ=255)tAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACTAACAACAAAGGCCTGGTATATATGAcgcgcg > 1:417900/1‑71 (MQ=255)tAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACTAACAACAAAGGCCTGGTATATATGAcgcgcg > 1:459382/1‑71 (MQ=255)tAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACTAACAACAAAGGCCTGGTATATATGAcgcgcg > 1:571313/1‑71 (MQ=255)tAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACTAACAACAAAGGCCTGGTATATATGAcgcgcg > 1:581362/1‑71 (MQ=255)tAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACTAACAACAAAGGCCTGGTATATATGAcgcgc > 1:438621/1‑70 (MQ=255)tAAAGCCAGCGTTGAAGACTGGGAAACGATGATT‑ATACTAACAACAAAGGCCTGGTATATATGACgcgcgc > 1:328383/1‑71 (MQ=255) | TAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGC > minE/979556‑979627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |