Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	984,909	C→A	100%	L80L (CTG→CTT)	ynfC ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	984,909	0	C	A	94.2%	167.3 / 4.6	52	L80L (CTG→CTT)	ynfC	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (49/0);  total (52/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTC  >  minE/984864‑984966                                              |                                                          atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTg                                              >  1:4218/1‑59 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:548322/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:123487/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:425514/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:434794/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:435865/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:443454/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:458601/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:479499/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:483816/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:516321/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:518618/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:547996/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:422628/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:566981/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:567100/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:567636/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:582506/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:599759/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:60306/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:60360/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:608672/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:619451/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:630725/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:648653/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:94252/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:315263/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:168919/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:200516/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:264546/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:266446/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:273300/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:280021/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:284103/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:309665/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:310267/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:314471/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:413122/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:323582/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:329549/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:333861/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:350638/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:350878/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:368871/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:38480/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:388796/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:389741/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:392130/1‑71 (MQ=255) atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCAAGTAATTCGAGTGAATCAAAACAGc                                  >  1:411865/1‑71 (MQ=255)                                 tattTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc  >  1:578450/1‑71 (MQ=255)                                 tattTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc  >  1:407324/1‑71 (MQ=255)                                 tattTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc  >  1:82815/1‑71 (MQ=255)                                              |                                                          ATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTC  >  minE/984864‑984966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑