Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,099,149	A→G	100%	T130A (ACA→GCA)	ydiM →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,099,149	0	A	G	100.0%	88.6 / NA	25	T130A (ACA→GCA)	ydiM	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/0);  total (25/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGC  >  minE/1099083‑1099153                                                                   |     gCAATCAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:90042/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:439508/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:96345/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:82538/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:72433/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:606182/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:605117/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:583572/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:56811/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:519292/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:517242/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:488699/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:46913/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:100157/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:432448/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:392393/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:328459/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:231351/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:208186/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:200251/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:19691/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:188398/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:16127/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc  >  1:129406/1‑71 (MQ=255) gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCAACGCTCACCTGGGGCAGc  >  1:243025/1‑71 (MQ=255)                                                                   |     GCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGC  >  minE/1099083‑1099153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑