Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,173,722	C→T	100%	Q310Q (CAG→CAA)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,173,722	0	C	T	100.0%	28.7 / NA	9	Q310Q (CAG→CAA)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/0);  total (9/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCG  >  minE/1173719‑1173789    |                                                                    gTTTTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAgcggcg  >  1:174360/1‑71 (MQ=255) gTTTTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAgcggcg  >  1:218025/1‑71 (MQ=255) gTTTTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAgcggcg  >  1:319047/1‑71 (MQ=255) gTTTTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAgcggcg  >  1:569465/1‑71 (MQ=255) gTTTTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAgcggcg  >  1:590323/1‑71 (MQ=255) gTTTTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAgcggc   >  1:138045/1‑70 (MQ=255) gTTTTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAgcggc   >  1:29395/1‑70 (MQ=255) gTTTTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAgcggc   >  1:347355/1‑70 (MQ=255) gTTTTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAgcggc   >  1:534515/1‑70 (MQ=255)    |                                                                    GTTCTGCGCACTCAGACCAAAACGTTTTGCCGCTTCAATCTGCAACGCTGAAAGCGAAAAAGGCAGCGGCG  >  minE/1173719‑1173789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑