Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,330,586	A→G	100%	Y334H (TAC→CAC)	cpsG ←	phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,330,586	0	A	G	100.0%	108.4 / NA	29	Y334H (TAC→CAC)	cpsG	phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/29);  total (0/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATA  >  minE/1330543‑1330613                                            |                            cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:405012/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:91021/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:89719/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:77725/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:639628/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:618250/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:548366/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:51910/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:518417/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:506403/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:444190/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:431565/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:426536/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:4182/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:118585/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:364298/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:358230/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:355763/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:34578/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:27654/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:276330/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:259798/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:210573/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:188040/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:18010/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:173267/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:160979/71‑1 (MQ=255) cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:151905/71‑1 (MQ=255)                     gCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa  <  1:492987/51‑1 (MQ=255)                                            |                            CACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATA  >  minE/1330543‑1330613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑