Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,509,579	G→A	100%	A42T (GCA→ACA)	ackA →	acetate kinase A and propionate kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,509,579	0	G	A	100.0%	100.1 / NA	27	A42T (GCA→ACA)	ackA	acetate kinase A and propionate kinase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/27);  total (0/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATA  >  minE/1509539‑1509609                                         |                               gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:122228/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:81378/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:78707/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:77216/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:569019/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:523652/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:507669/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:470502/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:443958/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:421413/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:412206/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:404181/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:372062/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:368180/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:343331/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:272183/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:271384/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:198793/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:198036/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:176304/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:162255/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:16134/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:132688/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:130638/71‑1 (MQ=255) gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:119185/71‑1 (MQ=255)  tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:99524/70‑1 (MQ=255)     cTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa  <  1:450853/67‑1 (MQ=255)                                         |                               GTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATA  >  minE/1509539‑1509609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑