| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,523,658 | C→T | 100% | Q153* (CAA→TAA) | flk → | predicted flagella assembly protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,523,658 | 0 | C | T | 100.0% | 20.6 / NA | 7 | Q153* (CAA→TAA) | flk | predicted flagella assembly protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/7); total (0/7) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCAC > minE/1523620‑1523664 | ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:205784/45‑1 (MQ=255)ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:234658/45‑1 (MQ=255)ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:392203/45‑1 (MQ=255)ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:4761/45‑1 (MQ=255)ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:556360/45‑1 (MQ=255)ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:557154/45‑1 (MQ=255) cTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:582640/37‑1 (MQ=255) | TTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCAC > minE/1523620‑1523664 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |