Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,664,529	G→A	100%	I231I (ATC→ATT)	guaA ←	GMP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,664,529	0	G	A	100.0%	302.4 / NA	80	I231I (ATC→ATT)	guaA	GMP synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (80/0);  total (80/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTTACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGT  >  minE/1664462‑1664578                                                                    |                                                  ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:263157/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:390763/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:375623/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:371645/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:369027/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:355290/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:339038/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:468563/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:298216/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:298029/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:502158/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:209087/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:552903/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:192212/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:560727/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:56809/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:134613/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:128843/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:173984/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:14834/1‑70 (MQ=255) ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:111005/1‑70 (MQ=255) ttatACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt                                                 >  1:280669/4‑70 (MQ=255)                                               caccCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:4942/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCTTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtc    >  1:193816/1‑69 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCt                                 >  1:196639/1‑40 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTAcc                              >  1:366671/1‑43 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcg                        >  1:588827/1‑49 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCgg                     >  1:350122/1‑52 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGCTGCGAg              >  1:148107/1‑59 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGc                 >  1:289131/1‑56 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGc                 >  1:473961/1‑56 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:660138/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:401732/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:86370/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:650628/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:429339/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:442/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:445753/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:446173/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:627330/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:46150/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:474990/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:47806/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:598938/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:481162/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:353357/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:536982/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:546932/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:573654/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:375636/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:105193/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:129922/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:146378/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:167085/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:170308/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:175636/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:203734/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:208647/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:221034/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:24409/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:292686/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:316764/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:381819/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:369725/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:360671/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:101198/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:349506/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:337489/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:337280/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:322752/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcg   >  1:349365/1‑70 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcg   >  1:519880/1‑70 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcg   >  1:278489/1‑70 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcg   >  1:395052/1‑70 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcg   >  1:652063/1‑70 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcg   >  1:307583/1‑70 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGACCTACAGCAtc    >  1:103035/1‑69 (MQ=255)                                               cacaCCACCAGAGAGGCCGAGAATGACTATATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgt  >  1:162812/1‑71 (MQ=255)                                               cacaCCACCAGAGAGGACGAGAATGACTTTATCGTCGCCTACCTGCt                          >  1:606631/1‑47 (MQ=255)                                               aacacCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcg   >  1:124006/2‑70 (MQ=255)                                                                    |                                                  TTTTACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGT  >  minE/1664462‑1664578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑