| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,000,743 | A→T | 100% | F180L (TTT→TTA) | gss ← | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,000,743 | 0 | A | T | 100.0% | 55.6 / NA | 16 | F180L (TTT→TTA) | gss | fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (0/16); total (0/16) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTC > minE/2000706‑2000775 | ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTGATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:655291/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:116678/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:170211/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:19290/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:24475/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:30540/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:331047/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:383564/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:38413/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:542076/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:546894/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:555167/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:564286/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:583753/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:625002/70‑1 (MQ=255)ccGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCTAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTc < 1:81079/70‑1 (MQ=255) | CCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTC > minE/2000706‑2000775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |