Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,017,565 C→T 100% E138E (GAG→GAA)  yqhC ← predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,017,5650CT100.0% 154.0 / NA 43E138E (GAG→GAA) yqhCpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/43);  total (0/43)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGCTCATCTTCGCC  >  minE/2017506‑2017576
                                                           |           
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:525984/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:354889/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:375477/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:424665/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:428499/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:475455/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:488543/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:504622/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:515493/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:524659/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:34605/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:568720/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:586024/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:603059/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:619094/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:626902/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:632504/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:644990/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:8497/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:9488/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:239007/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:105020/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:107593/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:108541/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:143467/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:202010/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:223798/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:230786/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:236287/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:344550/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:250595/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:27538/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:27713/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:28532/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:291004/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:31074/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:313154/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:316989/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:324525/71‑1 (MQ=255)
aTCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCAGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:102450/71‑1 (MQ=255)
                 ggCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:532143/54‑1 (MQ=255)
                    ggAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:178407/51‑1 (MQ=255)
                                    gctgCCATCGACGGCTGGAAATGTTCATCTTCGcc  <  1:633681/35‑1 (MQ=255)
                                                           |           
ATCTCTTCTGATAACGTGGCGGAGTTAATCCCGCTGGCTGCCATCGACGGCTGGAAATGCTCATCTTCGCC  >  minE/2017506‑2017576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑