| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,134,402 | T→C | 100% | E643E (GAA→GAG) | pnp ← | polynucleotide phosphorylase/polyadenylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,134,402 | 0 | T | C | 90.9% | 64.5 / ‑0.4 | 22 | E643E (GAA→GAG) | pnp | polynucleotide phosphorylase/polyadenylase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (20/0); total (20/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAAATGCGCCAAAGTCAACGATACGGGTCACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCG > minE/2134347‑2134466 | caaATGCGCCAAAGTCAACGATACGGGTCACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTg < 1:257536/71‑1 (MQ=255)caaATGCGCCAAAGTCAACGATACGGGTCACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTg < 1:344016/71‑1 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATacg > 1:21932/1‑36 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:194700/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:634033/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:630674/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:555491/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:553688/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:544866/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:524542/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:516218/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:506679/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:49113/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:401698/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:30864/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:216184/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:210575/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtcg > 1:146991/1‑71 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtc > 1:489395/1‑70 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtc > 1:410831/1‑70 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtc > 1:307098/1‑70 (MQ=255) gCCCACCTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCgtcggtc > 1:557668/1‑70 (MQ=255) | CAAATGCGCCAAAGTCAACGATACGGGTCACTTTACCAGTGTAGACGCGGCCCACTTCGATTTCTGCAGTGATCTCTTCGATACGACGAATAGCATGTTTCGCTTTCTCGCCGTCGGTCG > minE/2134347‑2134466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |