Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,187,308	T→C	100%	P183P (CCA→CCG)	nanT ←	sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,187,308	0	T	C	100.0%	98.2 / NA	27	P183P (CCA→CCG)	nanT	sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/27);  total (0/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAG  >  minE/2187257‑2187326                                                    |                   ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCTATAAAg  <  1:85/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:384491/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:643845/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:616813/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:658461/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:577643/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:500044/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:440352/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:439803/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:78997/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:433782/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:408150/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:361879/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:328195/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:30513/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:294342/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:251486/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:246203/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:88459/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:127351/70‑1 (MQ=255) ccAGTCTTCCGCTTCCGGGATGTTTGTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:209661/70‑1 (MQ=255) ccAGTCTTCCCCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAACTGCCGATAAAg  <  1:197278/70‑1 (MQ=255)  cAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:153598/69‑1 (MQ=255)  cAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:583412/69‑1 (MQ=255)  cAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:225887/69‑1 (MQ=255)              tccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:417815/57‑1 (MQ=255)                        ttttACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAg  <  1:43414/47‑1 (MQ=255)                                                    |                   CCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAG  >  minE/2187257‑2187326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑