| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,285,340 | C→A | 100% | L66L (CTG→CTT) | argH ← | argininosuccinate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,285,340 | 0 | C | A | 95.7% | 74.1 / ‑2.3 | 23 | L66L (CTG→CTT) | argH | argininosuccinate lyase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (0/22); total (1/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCCAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTGCTCTTCTGCGGTTAACACGCCTACCGTGAC > minE/2285306‑2285405 | cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:399204/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:97158/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:597811/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:593186/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:580641/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:571803/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:53690/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:498306/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:45325/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:109034/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:343818/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:281810/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:230337/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:217938/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:215126/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:194471/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:172981/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:171165/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:135914/70‑1 (MQ=255)cAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:10944/70‑1 (MQ=255) aaGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:641466/69‑1 (MQ=255) aaGGATTTGTTGTGGCCTGGCGCGAACATCTTCAAGCAACAAGTTCAGCGCCTCTTCCAGTTGCGCCTg < 1:644205/69‑1 (MQ=255) ttCCAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTGCTCTTCTGCGGTTAACACGCCTACCGTGAc > 1:531481/1‑69 (MQ=255) | CAAGGATTTGTTGTGGCCTGGCGCGAACATCTTCCAGCAACACGTTCAGCGCCTCTTCCAGTTGCGCCTGCTCTTCTGCGGTTAACACGCCTACCGTGAC > minE/2285306‑2285405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |