Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,334,270	G→A	100%	L573L (CTG→CTA)	fdoG →	formate dehydrogenase‑O, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,334,270	0	G	A	100.0%	151.2 / NA	41	L573L (CTG→CTA)	fdoG	formate dehydrogenase‑O, large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/41);  total (0/41)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTA  >  minE/2334252‑2334322                   |                                                     cataGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:474303/68‑1 (MQ=255) caaaGTTATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:235699/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGGAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:386284/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:573116/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:388814/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:38984/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:433408/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:478880/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:482622/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:500411/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:511284/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:525457/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:95750/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:596741/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:600791/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:602242/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:605160/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:609115/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:612542/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:657351/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:110260/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:244340/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:113591/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:157558/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:172057/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:181739/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:182610/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:19165/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:192018/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:24082/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:356993/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:263578/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:268499/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:27197/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:275306/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:277796/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:312149/71‑1 (MQ=255) caaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:319049/71‑1 (MQ=255) caaaGTGATCGGATGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:566569/71‑1 (MQ=255)  aaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:253660/70‑1 (MQ=255)  aaaGTGATCGGTTGTCTATCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTa  <  1:166762/70‑1 (MQ=255)                tctatcGTAACTGAAGTTCCTCGTCACCATCGACCCACTTAACACTGAAACCTCTa  <  1:407914/56‑1 (MQ=255)                   |                                                     CAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTA  >  minE/2334252‑2334322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑