Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	434,571	A→T	100%	W66R (TGG→AGG)	fldA ←	flavodoxin 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	434,571	0	A	T	100.0%	78.8 / NA	22	W66R (TGG→AGG)	fldA	flavodoxin 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/22);  total (0/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAG  >  minE/434567‑434637     |                                                                   tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:415190/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:88771/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:72200/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:644954/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:583217/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:55056/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:547956/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:484546/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:465028/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:458507/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:130090/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:412328/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:406834/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:346673/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:327817/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:311288/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:291534/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:270319/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:148471/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:14124/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCAGGCTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:302612/71‑1 (MQ=255) tCCCTGTCACACTGCGCTTCGCCGTAATACCACGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAg  <  1:87631/71‑1 (MQ=255)     |                                                                   TCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAG  >  minE/434567‑434637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑