| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,398,903 | T→C | 100% | E576E (GAA→GAG) | uvrD ← | DNA‑dependent ATPase I and helicase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,398,903 | 0 | T | C | 100.0% | 13.5 / NA | 5 | E576E (GAA→GAG) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (5/0); total (5/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGCTTGGGAACATGCCCTCTTCCATACCAACGATAAA > minE/2398883‑2398920 | tGGCTTGGGAACATGCCCTCCTCCATACCAACGATaaa > 1:225632/1‑38 (MQ=255)tGGCTTGGGAACATGCCCTCCTCCATACCAACGATaaa > 1:279292/1‑38 (MQ=255)tGGCTTGGGAACATGCCCTCCTCCATACCAACGATaaa > 1:335220/1‑38 (MQ=255)tGGCTTGGGAACATGCCCTCCTCCATACCAACGATaaa > 1:368397/1‑38 (MQ=255)tGGCTTGGGAACATGCCCTCCTCCATACCAACGATaaa > 1:439037/1‑38 (MQ=255) | TGGCTTGGGAACATGCCCTCTTCCATACCAACGATAAA > minE/2398883‑2398920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |