Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,516,656	C→T	100%	A102T (GCT→ACT)	trmH ←	tRNA (Guanosine‑2'‑O‑)‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,516,656	0	C	T	100.0%	267.5 / NA	70	A102T (GCT→ACT)	trmH	tRNA (Guanosine‑2'‑O‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (70/0);  total (70/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGC  >  minE/2516610‑2516677                                               |                      aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACTGTGTTATCAGAAAGATGGGTTGc  >  1:544297/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAg               >  1:295766/1‑55 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGaa             >  1:569135/1‑57 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAg           >  1:545030/1‑59 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGAt         >  1:283963/1‑61 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTg   >  1:502486/1‑67 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTg   >  1:307793/1‑67 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTg   >  1:59623/1‑67 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTg   >  1:329867/1‑67 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:4/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:390712/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:38903/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:403480/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:420566/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:372025/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:439621/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:371444/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:466865/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:470096/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:491370/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:525443/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:492922/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:524200/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:122272/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:530198/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:533813/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:537164/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:550212/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:558111/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:562501/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:579339/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:592761/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:600671/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:607527/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:627693/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:642957/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:7719/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:326896/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:121327/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:149233/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:151233/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:153733/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:157645/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:159502/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:160944/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:165968/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:180797/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:195436/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:202878/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:210857/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:215428/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:220591/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:239222/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:243165/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:335252/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:314314/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:314300/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:313581/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:290987/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:289603/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:288349/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:283093/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:267819/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:26539/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:265349/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:259697/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:252618/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:252215/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc  >  1:243069/1‑68 (MQ=255) aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTACCAGAAAGATGGGTTGc  >  1:86889/1‑68 (MQ=255)                                               |                      AAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGC  >  minE/2516610‑2516677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑