| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 451,791 | C→T | 100% | L81L (TTG→TTA) | kdpA ← | potassium translocating ATPase, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 451,791 | 0 | C | T | 100.0% | 12.9 / NA | 5 | L81L (TTG→TTA) | kdpA | potassium translocating ATPase, subunit A |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/0); total (5/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAG > minE/451744‑451814 | aGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGTAACATAAAAAACAGCACCGc > 1:645507/1‑68 (MQ=255)aGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGTAACATAAAAAACAGCACCGCCAg > 1:133444/1‑71 (MQ=255)aGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGTAACATAAAAAACAGCACCGCCAg > 1:14495/1‑71 (MQ=255)aGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGTAACATAAAAAACAGCACCGCCAg > 1:219465/1‑71 (MQ=255)aGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGTAACATAAAAAACAGCACCGCCAg > 1:314927/1‑71 (MQ=255) | AGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAG > minE/451744‑451814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |